Jiménez-Anguiano A, Báez-Saldaña A, Drucker-Colín R
Departamento de Neurociencias, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F.
Brain Res. 1993 Dec 24;631(2):345-8. doi: 10.1016/0006-8993(93)91556-8.
Intraventricular administration of cerebrospinal fluid (CSF) obtained from sleep deprived (SD) animals and vasoactive intestinal peptide (VIP) have been shown to increase rapid eye movement (REM) sleep. It has thus been suggested that VIP may accumulate in the CSF as a consequence of waking, and might thus be partly responsible for the subsequent rebound of REM sleep which follows prolonged wakefulness. To this data there are no studies testing this hypothesis. The purpose of this study, therefore, was to determine REM rebound following the extraction of CSF immediately after REMSD and to quantify by radioimmunoassay (RIA) the concentration of VIP in the CSF of progressively increasing REMSD periods. The results showed that REM rebound normally seen following REMSD is reduced by extraction of CSF, and that VIP concentration in such CSF is augmented. The results are discussed in terms of the possibility that waking induces an accumulation of VIP in the CSF, which is in turn involved in the production of REM sleep.
从睡眠剥夺（SD）动物获取的脑脊液（CSF）进行脑室内注射以及血管活性肠肽（VIP）已被证明可增加快速眼动（REM）睡眠。因此有人提出，VIP可能因清醒而在脑脊液中积累，从而可能部分导致长时间清醒后随后出现的REM睡眠反弹。对于这一数据，尚无研究检验这一假设。因此，本研究的目的是确定REM睡眠剥夺（REMSD）后立即抽取脑脊液后的REM反弹情况，并通过放射免疫分析（RIA）定量逐渐增加的REMSD时间段脑脊液中VIP的浓度。结果表明，REMSD后通常出现的REM反弹因抽取脑脊液而减少，且此类脑脊液中VIP浓度增加。就清醒诱导脑脊液中VIP积累进而参与REM睡眠产生的可能性对结果进行了讨论。
