Brooks S E, Kaza V, Nakamura T, Trousdale M D
Department of Ophthalmology, Los Angeles County-University of Southern California Medical Center.
Cornea. 1994 Jan;13(1):43-50. doi: 10.1097/00003226-199401000-00008.
Rose bengal and fluorescein are photosensitive dyes in widespread use in the evaluation of ocular surface diseases, including herpes simplex virus (HSV) keratitis. These dyes have recently been shown to penetrate living cells, and rose bengal was previously reported to possess antiviral activity. Several experiments reported herein suggest that these dyes do possess the potential for potent antiviral activity against extracellular virus, but only in the presence of light. Rose bengal is substantially more effective in vitro than fluorescein, and the effect is greater with increasing concentration of dye and duration of light exposure. Electron microscopic evaluation of treated virus showed no structural difference from untreated virus, in spite of 4- to 5-log decreases in virus titer. Intracellular virus was found to be markedly resistant to photoinactivation. In a rabbit model of acute primary HSV keratitis, daily application of topical rose bengal followed by light exposure had no therapeutic effect, although an adverse effect on culture sensitivity testing was seen.
孟加拉玫瑰红和荧光素是广泛用于评估眼表疾病（包括单纯疱疹病毒性角膜炎）的光敏染料。最近已证明这些染料可穿透活细胞，并且先前报道孟加拉玫瑰红具有抗病毒活性。本文报道的几项实验表明，这些染料确实具有对细胞外病毒产生强效抗病毒活性的潜力，但仅在有光的情况下才会如此。孟加拉玫瑰红在体外比荧光素有效得多，并且随着染料浓度的增加和光照时间的延长，效果会更显著。尽管病毒滴度降低了4至5个对数，但对经处理病毒的电子显微镜评估显示其结构与未处理病毒没有差异。发现细胞内病毒对光灭活具有明显抗性。在急性原发性单纯疱疹病毒性角膜炎的兔模型中，每天局部应用孟加拉玫瑰红后再进行光照没有治疗效果，尽管观察到对培养敏感性测试有不良影响。
