Feenstra R P, Tseng S C
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, FL 33101.
Ophthalmology. 1992 Apr;99(4):605-17. doi: 10.1016/s0161-6420(92)31947-5.
The authors have recently reported that rose bengal is not a vital dye, and stains whenever the cultured cells are not covered by such components as albumin and mucin, and such a tear substitute as carboxycellulose. In this report, using cultured cells as well as normal rabbit corneas, they characterize and correlate the staining differences between rose bengal and fluorescein with the differences in their chemical structures. Fluorescein differs from rose bengal in its lack of intrinsic toxicity, photodynamic action, and ability to be blocked by the above-mentioned substances. Fluorescein staining is increased by rapid stromal diffusion and hence can manifest whenever there is disruption of cell-cell junctions. In contrast, rose bengal staining ensues whenever there is deficiency of preocular tear film protection. These experimental data may help interpret the clinical staining properties of these two dyes and enhance the understanding of the pathogenesis of various ocular surface disorders.
作者们最近报道，孟加拉玫瑰红并非一种活体染料，只要培养的细胞未被白蛋白、黏蛋白等成分以及羧甲基纤维素等泪液替代物覆盖，它就会产生染色。在本报告中，他们使用培养的细胞以及正常兔角膜，对孟加拉玫瑰红和荧光素之间的染色差异进行了表征，并将其与它们化学结构上的差异相关联。荧光素与孟加拉玫瑰红的不同之处在于其缺乏内在毒性、光动力作用以及被上述物质阻断的能力。荧光素染色会因基质快速扩散而增加，因此只要细胞间连接遭到破坏就会显现出来。相比之下，只要眼前泪膜保护不足，就会出现孟加拉玫瑰红染色。这些实验数据可能有助于解释这两种染料的临床染色特性，并增进对各种眼表疾病发病机制的理解。
