Roat M I, Romanowski E, Araullo-Cruz T, Gordon Y J
Eye and Ear Institute, University of Pittsburgh, PA 15213.
Arch Ophthalmol. 1987 Oct;105(10):1415-7. doi: 10.1001/archopht.1987.01060100117039.
We evaluated the antiviral effects of rose bengal and fluorescein sodium. The direct antiviral activity was determined by an in vitro direct neutralization assay. The 50% inhibitory dose was 16 micrograms/mL for rose bengal and 460 micrograms/mL for fluorescein. The in vivo antiviral effects of these drugs were determined in the mouse herpetic keratitis model. Following topical application, rose bengal reduced surface virus titers (swabs) 1 million-fold, and residual ocular virus (eye homogenates) 32-fold, compared with controls. No infectious virus was recovered by swabbing after topical application of rose bengal. Fluorescein had no significant effect on virus replication. Thus, rose bengal, unlike fluorescein, has significant antiviral activity, and the diagnostic use of rose bengal prior to viral culture may preclude a positive result. Also, the use of rose bengal to grade keratitis in the study of new antiviral agents should be discouraged.
我们评估了孟加拉玫瑰红和荧光素钠的抗病毒作用。通过体外直接中和试验测定直接抗病毒活性。孟加拉玫瑰红的50%抑制剂量为16微克/毫升，荧光素为460微克/毫升。在小鼠疱疹性角膜炎模型中测定了这些药物的体内抗病毒作用。局部应用后，与对照组相比，孟加拉玫瑰红使表面病毒滴度（拭子）降低了100万倍，残余眼部病毒（眼匀浆）降低了32倍。局部应用孟加拉玫瑰红后，拭子未检出感染性病毒。荧光素对病毒复制无显著影响。因此，与荧光素不同，孟加拉玫瑰红具有显著的抗病毒活性，在病毒培养前使用孟加拉玫瑰红进行诊断可能会导致结果为阴性。此外，在新抗病毒药物研究中，不应鼓励使用孟加拉玫瑰红对角膜炎进行分级。
