Cordell J L, Pulford K, Turley H, Jones M, Micklem K, Doussis I A, Tyler X, Mayne K, Gatter K C, Mason D Y
Nuffield Department of Pathology, John Radcliffe Hospital, Oxford.
J Clin Pathol. 1994 Feb;47(2):143-7. doi: 10.1136/jcp.47.2.143.
To describe the distribution of the recently cloned human leucocyte adhesion molecule ICAM-3 in normal and neoplastic tissues and cell lines.
A panel of four monoclonal antibodies to ICAM-3 were used to stain cell lines and sections of human lymphoid tissues using the alkaline phosphatase-anti-alkaline phosphatase immunocytochemical method (APAAP).
In peripheral blood ICAM-3 was detected on monocytes, granulocytes, and most lymphocytes. In sections of human lymphoid tissue the antigen was also found on most lymphocytes, but many of the proliferating B cells found in the germinal centres of secondary lymphoid follicles were ICAM-3 negative. ICAM-3 was also found on neoplastic white cells (in chronic lymphocytic leukaemia, hairy cell leukaemia, acute and chronic myeloid leukaemia, and multiple myeloma) with the exception of Reed-Sternberg cells in Hodgkin's disease, many of which were negative. ICAM-3 was consistently absent from cells and tissues of non-haemopoietic origin. Endothelium (which expresses ICAM-1) was negative for ICAM-3, with the exception of vessels in some neoplastic lymphoid samples which showed variable staining for ICAM-3.
These findings suggest that ICAM-3 is essentially restricted to the haemopoietic system and is reciprocal in its expression to ICAM-1, in that it is present on resting cells and its level falls as a result of cell activation.
描述最近克隆的人类白细胞粘附分子ICAM - 3在正常组织、肿瘤组织及细胞系中的分布情况。
使用一组针对ICAM - 3的四种单克隆抗体，采用碱性磷酸酶 - 抗碱性磷酸酶免疫细胞化学方法（APAAP）对细胞系及人类淋巴组织切片进行染色。
在外周血中，ICAM - 3在单核细胞、粒细胞及大多数淋巴细胞上被检测到。在人类淋巴组织切片中，该抗原也存在于大多数淋巴细胞上，但在次级淋巴滤泡生发中心发现的许多增殖性B细胞为ICAM - 3阴性。ICAM - 3也存在于肿瘤白细胞中（慢性淋巴细胞白血病、毛细胞白血病、急性和慢性髓细胞白血病以及多发性骨髓瘤），但霍奇金病中的里德 - 斯腾伯格细胞除外，其中许多为阴性。非造血来源的细胞和组织中始终不存在ICAM - 3。内皮细胞（表达ICAM - 1）对ICAM - 3呈阴性，除了一些肿瘤淋巴样本中的血管对ICAM - 3呈现可变染色。
这些发现表明ICAM - 3基本上局限于造血系统，并且其表达与ICAM - 1相反，即它存在于静息细胞上，并且其水平会因细胞活化而下降。
