临床样本中传染性HIV的稳定性及从小体积全血中的分离
Stability of infectious HIV in clinical samples and isolation from small volumes of whole blood.
作者信息
O'Shea S, Rostron T, Mullen J E, Banatvala J E
机构信息
Department of Virology United Medical Schools, Guys Hospital, London.
出版信息
J Clin Pathol. 1994 Feb;47(2):152-4. doi: 10.1136/jcp.47.2.152.
AIMS
To evaluate the stability of infectious HIV in clinical samples and the efficiency of isolating it from small volumes of whole blood.
METHODS
Titres of infectious HIV were measured in peripheral blood mononuclear cells and plasma 2, 24, and 48 hours after peripheral blood had been collected from 10 HIV positive adult volunteers. Volumes of whole blood (1 ml to 5 microliters), collected from a further five volunteers, were used to determine the minimum volume from which HIV could be isolated. Infectious HIV was isolated by co-culture with phytohaemagglutinin stimulated umbilical cord mononuclear cells.
RESULTS
Geometric mean titres of infectious HIV seemed to be more stable in peripheral blood mononuclear cells than in plasma. HIV was recovered from all 10 peripheral blood mononuclear cell samples during the 48 hours after sample collection, but from only four plasma samples. HIV could occasionally be isolated from 5 microliters of whole blood and reliably from 200 microliters.
CONCLUSIONS
HIV can be isolated from peripheral blood mononuclear cells and plasma for up to 48 hours after sample collection. Isolation of HIV from small volumes of whole blood has applications for the diagnosis and management, of HIV positive children.
目的
评估临床样本中传染性HIV的稳定性以及从小体积全血中分离HIV的效率。
方法
从10名HIV阳性成年志愿者采集外周血后2小时、24小时和48小时，检测外周血单个核细胞和血浆中传染性HIV的滴度。从另外5名志愿者采集不同体积（1毫升至5微升）的全血，以确定能够分离出HIV的最小体积。通过与植物血凝素刺激的脐带单个核细胞共培养来分离传染性HIV。
结果
传染性HIV的几何平均滴度在外周血单个核细胞中似乎比在血浆中更稳定。在样本采集后的48小时内，从所有10份外周血单个核细胞样本中都能回收HIV，但仅从4份血浆样本中回收。偶尔能从5微升全血中分离出HIV，而从200微升全血中则能可靠地分离出。
结论
样本采集后长达48小时，可从外周血单个核细胞和血浆中分离出HIV。从小体积全血中分离HIV对HIV阳性儿童的诊断和管理有应用价值。
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