May G L, Sztelma K, Sorrell T C
Center for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, NSW Australia.
Magn Reson Med. 1994 Feb;31(2):212-7. doi: 10.1002/mrm.1910310215.
Stimulation of human peripheral blood neutrophils with lipopolysaccharide (LPS), arachidonic acid (AA) and oleic acid (OA) resulted in significant increases in cytoplasmic lipid droplets. This phenomenon was also observed in enucleated and degranulated cytoplasts prepared from neutrophils stimulated with LPS. In contrast, only LPS and high concentrations of OA (10 microM) produced an increase in the lipid intensities of the MR spectra of neutrophils as determined by COSY cross peak volume measurements. Lipid intensities in cells stimulated with OA (2.5 microM) and AA (2.5 microM) and phorbol myristate acetate (20 nM) were not elevated. LPS stimulation of resting cytoplasts resulted in increased lipid droplets but not MR lipid intensities. These data suggest that while cytoplasmic lipid droplets may correlate with MR lipid intensity under some circumstances, their presence is not sufficient to account for increased neutral lipid signals.
用脂多糖（LPS）、花生四烯酸（AA）和油酸（OA）刺激人外周血中性粒细胞会导致细胞质脂滴显著增加。在用LPS刺激的中性粒细胞制备的去核和脱粒细胞质体中也观察到了这种现象。相比之下，通过COSY交叉峰体积测量确定，只有LPS和高浓度的OA（10微摩尔）会使中性粒细胞的磁共振波谱脂质强度增加。用OA（2.5微摩尔）、AA（2.5微摩尔）和佛波酯（20纳摩尔）刺激的细胞中的脂质强度没有升高。LPS刺激静止的细胞质体会导致脂滴增加，但不会使磁共振脂质强度增加。这些数据表明，虽然在某些情况下细胞质脂滴可能与磁共振脂质强度相关，但其存在不足以解释中性脂质信号的增加。
