通过19F-和31P-核磁共振鉴别N-417小细胞肺癌细胞提取物中的氟化尿苷代谢物。

Discrimination of fluorinated uridine metabolites in N-417 small cell lung cancer cell extracts via 19F- and 31P-NMR.

作者信息

Pederson N V, Zanghi J A, Miller W M, Knop R H

机构信息

Chemical Engineering Department, Northwestern University, Evanston, Illinois.

出版信息

Magn Reson Med. 1994 Feb;31(2):224-8. doi: 10.1002/mrm.1910310217.

DOI:10.1002/mrm.1910310217

PMID:8133759

Abstract

31P-NMR extract spectra of N-417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP-glucose, FUDP-glucuronate, FUDP-GlcNAc, and FUDP-GalNAc via enzymatic conversion and 19F- and 31P-NMR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP-sugars.

摘要

用氟尿苷（FUrd）培养的N - 417小细胞肺癌（SCLC）细胞的31P - NMR提取物光谱显示，在二磷酸二酯和核苷三磷酸区域出现了具有化学位移的新峰。通过酶促转化以及19F - 和31P - NMR分析，这些峰被鉴定为氟尿苷三磷酸（FUTP）、氟尿苷二磷酸（FUDP）、氟尿苷二磷酸葡萄糖（FUDP - 葡萄糖）、氟尿苷二磷酸葡萄糖醛酸（FUDP - 葡萄糖醛酸）、氟尿苷二磷酸N - 乙酰葡糖胺（FUDP - GlcNAc）和氟尿苷二磷酸N - 乙酰半乳糖胺（FUDP - GalNAc）。为FUTP、FUDP和FUDP - 糖类指定了不同的19F化学位移。