Takeishi M, Hirase Y, Kojima T
Department of Plastic and Reconstructive Surgery, Jikei University School of Medicine, Tokyo, Japan.
Microsurgery. 1994;15(1):55-62. doi: 10.1002/micr.1920150114.
It has long been known that the antigenicity of tissues is depressed when they are kept at a very low temperature. In this study, Lewis rats and Brown Norway (B.N.) rats were used because these species are significantly dissimilar at the major histocompatibility complex (MHC) Femoral arteries were harvested from Lewis rats, frozen while lowering the temperature in a stepwise fashion to -70 degrees C. After three weeks of cryogenic storage, the arteries were transplanted into Lewis and B.N. rats. At various times from the first to the twelfth week after surgery, grafted vessels were observed, and pathological and electron microscopic studies were carried out. The vessels were found to be in good condition at the end of the 12 week observation period. These results strongly suggest that prolonged cryopreservation of vessels makes allotransplantation without immunosuppression possible.
长期以来，人们一直知道，当组织保存在非常低的温度下时，其抗原性会降低。在本研究中，使用了刘易斯大鼠和棕色挪威（B.N.）大鼠，因为这些物种在主要组织相容性复合体（MHC）上有显著差异。从刘易斯大鼠身上采集股动脉，在逐步降温至-70摄氏度的同时进行冷冻。经过三周的低温保存后，将动脉移植到刘易斯大鼠和B.N.大鼠体内。在手术后第一周至第十二周的不同时间，观察移植的血管，并进行病理和电子显微镜研究。在12周观察期结束时，发现血管状况良好。这些结果有力地表明，血管的长期冷冻保存使得无需免疫抑制的同种异体移植成为可能。
