Kawamoto H, Hasegawa S, Sawatari S, Miwa C, Morita O, Hosokawa T, Tanaka H
Department of Health Science, Gifu Prefectural Health and Environment Research Center, Japan.
Microbiol Immunol. 1993;37(12):991-7. doi: 10.1111/j.1348-0421.1993.tb01736.x.
Two outbreaks of non-bacterial gastroenteritis occurred in Gifu prefecture in January 1989 and in January 1991. Both outbreaks were closely related to the consumption of raw oysters, and showed similar clinical features. Small, round-structured virus particles were found in patient stools in both outbreaks by electron microscopy. The role of these particles as the causative agents of the outbreaks were strongly suggested by immune electron microscopy and/or western-blotting immunoassay. When compared with SRSV-9 (Tokyo/SRSV/86-510) reported previously (Hayashi et al, J. Clin. Microbiol., 27: 1728-1733, 1989), it was found that these viral particles were antigenically similar to SRSV-9, and had a major structural protein of 63 kilodaltons (kDa). Further, the prevalence of this agent in Gifu area was examined by western blot antibody assay using 67 serum samples collected from the inhabitants in 1991. The results indicated the circulation of the same or antigenically similar agent in this area.
1989年1月和1991年1月，岐阜县发生了两起非细菌性肠胃炎疫情。这两起疫情均与食用生牡蛎密切相关，且临床表现相似。通过电子显微镜检查，在两起疫情患者的粪便中均发现了小的、圆形结构的病毒颗粒。免疫电子显微镜和/或蛋白质印迹免疫分析有力地表明了这些颗粒是疫情的病原体。与之前报道的SRSV - 9（东京/SRSV/86 - 510）（林等人，《临床微生物学杂志》，27: 1728 - 1733，1989年）相比，发现这些病毒颗粒在抗原性上与SRSV - 9相似，并且有一种主要结构蛋白，分子量为63千道尔顿（kDa）。此外，1991年使用从当地居民收集的67份血清样本，通过蛋白质印迹抗体分析检测了该病原体在岐阜地区的流行情况。结果表明该地区存在相同或抗原性相似的病原体传播。
