Masuzawa N, Togashi S, Itoh N, Kobayashi T, Inazuki G, Fujimaki M, Nakamura H, Nakayama H
Department of Psychiatry, Niigata University School of Medicine, Japan.
Neurosci Res. 1993 Oct;18(1):27-34. doi: 10.1016/0168-0102(93)90102-v.
Anti-idiotypic monoclonal antibodies that interact with the binding site of sigma receptors were generated. First, BALB/c mice were immunized with a haloperidol-bovine serum albumin conjugate, and monoclonal anti-haloperidol antibodies that recognize the piperidinyl moiety of haloperidol molecule were obtained. Second, for generation of anti-idiotypic antibodies, BALB/c mice were immunized with the anti-haloperidol monoclonal antibodies coupled to keyhole limpet hemocyanin. Anti-idiotypic antisera and three hybridomas secreting anti-idiotypic monoclonal antibodies were obtained. All of them were shown to inhibit [3H]haloperidol binding to the anti-haloperidol antibodies. The anti-idiotypes were potent in displacing the binding of [3H]haloperidol to rat brain sigma receptors. Furthermore, they significantly immunoprecipitated the sigma receptors from a detergent-solubilized preparation. These findings demonstrate the generation of anti-idiotypic monoclonal antibodies specifically interacting with membrane-bound and solubilized sigma receptors.
制备了与σ受体结合位点相互作用的抗独特型单克隆抗体。首先，用氟哌啶醇 - 牛血清白蛋白偶联物免疫BALB/c小鼠，获得了识别氟哌啶醇分子哌啶基部分的抗氟哌啶醇单克隆抗体。其次，为了产生抗独特型抗体，用与钥孔血蓝蛋白偶联的抗氟哌啶醇单克隆抗体免疫BALB/c小鼠。获得了抗独特型抗血清和三种分泌抗独特型单克隆抗体的杂交瘤。所有这些都显示出抑制[3H]氟哌啶醇与抗氟哌啶醇抗体的结合。这些抗独特型抗体在取代[3H]氟哌啶醇与大鼠脑σ受体的结合方面很有效。此外，它们从去污剂溶解的制剂中显著免疫沉淀了σ受体。这些发现证明了特异性与膜结合和溶解的σ受体相互作用的抗独特型单克隆抗体的产生。
