Asano R, Murasugi E, Hokari S
Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Zentralbl Veterinarmed A. 1993 Nov-Dec;40(9-10):641-5. doi: 10.1111/j.1439-0442.1993.tb00679.x.
A method for the fractionation of swine erythrocytes according to age using Percoll is described. Centrifugation of erythrocytes on discontinuous Percoll gradients yielded four fractions of erythrocytes. To ascertain that each fraction of erythrocytes represented a different age group, the activities of hexokinase (Hx), aldolase, glyceraldehyde-3-phosphate dehydrogenase (GAPD), pyruvate kinase (PK), lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) were determined. These enzyme activities decreased successively from the top to the bottom fractions of the centrifuged column. Young erythrocytes obtained from the upper fractions of the centrifuged column exhibited a higher activity of each enzyme than that found in the heavier and older erythrocytes at the bottom fraction. This method is proposed as the most appropriate for use as an aid in distinguishing the presence of a young erythrocyte population.
描述了一种使用Percoll根据年龄对猪红细胞进行分级分离的方法。在不连续的Percoll梯度上对红细胞进行离心，得到了四个红细胞级分。为确定每个红细胞级分代表不同的年龄组，测定了己糖激酶（Hx）、醛缩酶、甘油醛-3-磷酸脱氢酶（GAPD）、丙酮酸激酶（PK）、乳酸脱氢酶（LDH）、葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）的活性。这些酶的活性从离心柱的顶部到底部级分依次降低。从离心柱上部级分获得的年轻红细胞，其每种酶的活性均高于底部较重、较老红细胞中的活性。该方法被认为是最适合用于辅助鉴别年轻红细胞群体存在的方法。
