Inaba N, Fukasawa I, Okajima Y, Ota Y, Tanaka K, Matsui H, Iwasaki H, Sato N, Sudo H, Björklund B
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Chiba, Japan.
Asia Oceania J Obstet Gynaecol. 1993 Dec;19(4):459-66. doi: 10.1111/j.1447-0756.1993.tb00407.x.
Using a monoclonal immunoradiometrical assay, we measured the concentrations of the specific epitope M3 of tissue polypeptide antigen (TPA), namely, tissue polypeptide specific antigen (TPS), in serum and amniotic fluid obtained from normal, healthy, nonpregnant and pregnant Japanese women (NHNJW and NHPJW). The cut-off value of the serum TPS level was set at 130 U/l, based on the mean +2 standard deviations in the NHNJW. The serial measurement of serum TPS levels demonstrated the significant temporal elevation of TPS level near the time of ovulation (+/- 3 days) in the ovulatory women. In the NHPJW, the mean serum TPS level and the positivity rate (> 130 U/l) increased with the advance of gestation, reaching 183.7 U/l and 66.7%, respectively, in the third trimester. Maternal serum levels were much elevated just before (mean: 982.3 U/l) and after (mean: 824.6 U/l) delivery, and were reduced to about one-fourth by 3 days. In addition, markedly high TPS concentrations were found in the retroplacental blood (one case: 9,076 U/l) and the amniotic fluid (mean: 11,650 U/l). The serum TPS level correlated well (r = 0.871) with the serum TPA level in the TPS range of 2-2,000 U/l, while a poor correlation (r = 0.273) was found in the TPS range of 2-500 U/l. The present study thus obtained fundamental data on TPS in Japanese women. The fluctuation and change of serum TPS levels during the menstrual cycle and in pregnancy should be taken into consideration when we apply TPS as a tumor marker for women.
我们使用单克隆免疫放射测定法，测量了从正常、健康、未怀孕和怀孕的日本女性（非怀孕未育日本女性和怀孕日本女性）获得的血清和羊水中组织多肽抗原（TPA）的特异性表位M3，即组织多肽特异性抗原（TPS）的浓度。基于非怀孕未育日本女性的均值加2个标准差，将血清TPS水平的临界值设定为130 U/l。对排卵女性血清TPS水平的连续测量表明，在排卵时间（±3天）附近，TPS水平出现显著的暂时性升高。在怀孕日本女性中，血清TPS的平均水平和阳性率（>130 U/l）随着孕周的增加而升高，在孕晚期分别达到183.7 U/l和66.7%。产妇血清水平在分娩前（平均：982.3 U/l）和分娩后（平均：824.6 U/l）大幅升高，并在3天后降至约四分之一。此外，在胎盘后血液（1例：9,076 U/l）和羊水中（平均：11,650 U/l）发现了明显高浓度的TPS。在2 - 2,000 U/l的TPS范围内，血清TPS水平与血清TPA水平相关性良好（r = 0.871），而在2 - 500 U/l的TPS范围内相关性较差（r = 0.273）。因此，本研究获得了日本女性TPS的基础数据。当我们将TPS用作女性肿瘤标志物时，应考虑月经周期和孕期血清TPS水平的波动和变化。
