Bammler T K, Smith C A, Wolf C R
Imperial Cancer Research Fund, Molecular Pharmacology Unit, Ninewells Hospital Medical School, Dundee, U.K.
Biochem J. 1994 Mar 1;298 ( Pt 2)(Pt 2):385-90. doi: 10.1042/bj2980385.
Pi-class glutathione S-transferases (GSTs) play an important role in the detoxification of chemical toxins and mutagens and are implicated in neoplastic development and drug resistance. In all species characterized to date, only one functional Pi-class GST gene has been described. In this report we have identified two actively transcribed murine Pi-class GST genes, Gst p-1 and Gst p-2. The coding regions of Gst p-1 and the mouse Pi-class GST cDNA (GST-II) reported by Hatayama, Satoh and Satoh (1990) (Nucleic Acids Res. 18, 4606) are identical, whereas Gst p-2 encodes a protein that has not been described previously. The two genes are approximately 3 kb long and contain seven exons interrupted by six introns. In addition to a TATA box and a sequence motif matching the phorbol-ester-responsive element, the promoters of Gst p-1 and Gst p-2 exhibit one and two G+C boxes (GGGCGG) respectively. The cDNAs of the two genes were isolated from total liver RNA using reverse PCR. The peptide sequence deduced from the cDNAs share 97% identity and differ in six amino acids. Both genes are transcribed at significantly higher levels in male mouse liver than in female, and Gst p-1 mRNA is more abundant in both sexes than Gst p-2.
π类谷胱甘肽S-转移酶（GSTs）在化学毒素和诱变剂的解毒过程中发挥重要作用，并与肿瘤发生和耐药性有关。在迄今已鉴定的所有物种中，仅描述了一个功能性π类GST基因。在本报告中，我们鉴定出两个活跃转录的小鼠π类GST基因，即Gst p-1和Gst p-2。Gst p-1的编码区与Hatayama、Satoh和Satoh（1990年）（《核酸研究》18卷，4606页）报道的小鼠π类GST cDNA（GST-II）相同，而Gst p-2编码一种此前未被描述过的蛋白质。这两个基因长度约为3 kb，包含7个外显子，被6个内含子隔开。除了一个TATA盒和一个与佛波酯反应元件匹配的序列基序外，Gst p-1和Gst p-2的启动子分别有一个和两个G+C盒（GGGCGG）。使用逆转录PCR从肝脏总RNA中分离出这两个基因的cDNA。从cDNA推导的肽序列有97%的同一性，在6个氨基酸上存在差异。这两个基因在雄性小鼠肝脏中的转录水平均显著高于雌性，并且Gst p-1 mRNA在两性中都比Gst p-2更丰富。
