Sasaki M, Nishio M, Sasaki T, Enami J
Research Laboratory, Zenyaku Kogyo Co., Ltd., Tokyo, Japan.
Biochem Biophys Res Commun. 1994 Mar 15;199(2):772-9. doi: 10.1006/bbrc.1994.1296.
A growth factor for normal and neoplastic mouse mammary epithelial cells in primary culture was partially purified from the conditioned medium (CM) of mouse mammary fibroblasts. The N-terminal amino acid sequence of the major 33 kD protein separated on SDS-polyacrylamide gel electrophoresis under reducing conditions suggested that the growth factor is closely related to hepatocyte growth factor (HGF). To examine whether mammary growth factor is identical to HGF we cloned mouse HGF cDNA from mammary fibroblasts using PCR with primers designed from the previously reported human and rat HGF cDNA sequences. We then expressed the cloned mouse HGF cDNA in COS-1 cells. The CM from mouse HGF cDNA-transfected COS-1 cells stimulated the growth of mammary tumor cells in a manner similar to the CM from mammary fibroblasts. From these observations it is concluded that mammary growth factor produced by mammary fibroblasts is identical to mouse HGF.
从小鼠乳腺成纤维细胞的条件培养基（CM）中部分纯化出一种用于原代培养的正常和肿瘤性小鼠乳腺上皮细胞的生长因子。在还原条件下，通过SDS-聚丙烯酰胺凝胶电泳分离出的主要33kD蛋白的N端氨基酸序列表明，该生长因子与肝细胞生长因子（HGF）密切相关。为了检测乳腺生长因子是否与HGF相同，我们使用根据先前报道的人和大鼠HGF cDNA序列设计的引物，通过PCR从小鼠乳腺成纤维细胞中克隆了小鼠HGF cDNA。然后，我们在COS-1细胞中表达克隆的小鼠HGF cDNA。来自转染了小鼠HGF cDNA的COS-1细胞的CM以类似于来自乳腺成纤维细胞的CM的方式刺激乳腺肿瘤细胞的生长。从这些观察结果得出结论，乳腺成纤维细胞产生的乳腺生长因子与小鼠HGF相同。
