Rowbottom A W, Riches P G, Downie C, Hobbs J R
Department of Immunology, Charing Cross and Westminster Medical School, London, UK.
Bone Marrow Transplant. 1993 Dec;12(6):635-41.
Plasma concentrations and peripheral blood cells containing cytoplasmic cytokines were monitored during the post-transplant period in 10 patients who had received allogeneic bone marrow transplants (BMT) for the correction of inherited genetic disorders. The presence of CD14-positive cells containing cytoplasmic interleukin-1 alpha and beta in the peripheral blood was indicative of acute graft-versus-host disease (GVHD). Plasma concentrations of IL-1 alpha, IL-1 beta and TNF-alpha were significantly raised in the GVHD group when compared with the uneventful days. There was, however, poor temporal correlation between the plasma concentrations and clinical manifestations of acute GVHD. Cells containing cytoplasmic IL-6 were present in the peripheral blood when patients had clinically suspected and/or microbiologically confirmed infection. The results from this study demonstrate that analysis of peripheral blood cells for cytoplasmic IL-1 alpha and IL-1 beta are better markers of acute GVHD than is monitoring plasma concentrations of these cytokines.
对10例因遗传性疾病接受异基因骨髓移植（BMT）的患者在移植后阶段监测血浆浓度以及含有细胞质细胞因子的外周血细胞。外周血中存在含有细胞质白细胞介素-1α和β的CD14阳性细胞提示急性移植物抗宿主病（GVHD）。与病情平稳的日子相比，GVHD组中白细胞介素-1α、白细胞介素-1β和肿瘤坏死因子-α的血浆浓度显著升高。然而，急性GVHD的血浆浓度与临床表现之间的时间相关性较差。当患者临床上怀疑有感染和/或微生物学确诊有感染时，外周血中存在含有细胞质白细胞介素-6的细胞。本研究结果表明，分析外周血细胞中的细胞质白细胞介素-1α和白细胞介素-1β比监测这些细胞因子的血浆浓度更能作为急性GVHD的标志物。
