Adamson R, Jones A S, Field J K
Department of Clinical Dental Sciences, School of Dentistry, University of Liverpool, UK.
Oncogene. 1994 Jul;9(7):2077-82.
In this study we have analysed 38 squamous cell carcinomas (SCC) two basal cell carcinomas (BCC), a malignant salivary gland carcinoma, a neurosarcoma and a Warthins carcinoma, all isolated from patients with head and neck cancers. These tumour types have been examined by PCR for loss of heterozygosity (LOH) on both arms of chromosome 17 using nine polymorphic microsatellite markers. LOH on 17p in SCCHN was found to be 50% (19/38), and often involved TP53 (42%) but more frequently involved the CHRNB1 locus (56%). Twelve tumours showed loss of heterozygosity on 17q (34%) and ten of these also had loss on 17p. Four SCCHN tumours lost an entire copy of chromosome 17. It was of particular note that 77% (10/13) of the SCCHN at the hypopharyngeal site had LOH at CHRNB1. We propose from our data that the 17p12-p11 region contains a novel predisposing gene for hypopharyngeal SCCHN that may function as a tumour suppressor gene.
在本研究中，我们分析了38例鳞状细胞癌（SCC）、2例基底细胞癌（BCC）、1例恶性唾液腺癌、1例神经肉瘤和1例沃辛瘤，所有这些肿瘤均取自头颈癌患者。使用9个多态性微卫星标记，通过聚合酶链反应（PCR）检测了这些肿瘤类型在17号染色体两条臂上的杂合性缺失（LOH）情况。在头颈部鳞状细胞癌（SCCHN）中，17p上的LOH发生率为50%（19/38），常累及TP53（42%），但更常累及CHRNB1基因座（56%）。12例肿瘤在17q上显示杂合性缺失（34%），其中10例在17p上也有缺失。4例SCCHN肿瘤丢失了整条17号染色体。特别值得注意的是，下咽部位的SCCHN中有77%（10/13）在CHRNB1处存在LOH。根据我们的数据，我们推测17p12 - p11区域包含一个新的下咽SCCHN易感基因，该基因可能作为肿瘤抑制基因发挥作用。
