Bergerson W, Amselgruber W, Sinowatz F, Bergerson M
Institute for Veterinary Anatomy II, University of Munich, Germany.
Cell Tissue Res. 1994 Mar;275(3):537-41. doi: 10.1007/BF00318822.
The present lectin histochemical comparison of cauda epididymal and ampullary bovine sperm was conducted to investigate whether ampullary secretions are involved in altering the plasma-membrane glycoconjugates of sperm. A marked redistribution of glycoconjugates between sperm from these two regions was indeed revealed on the basis of changes in binding patterns of the following fluoroscein-isothiocyanate (FITC)-labelled lectins: wheat-germ agglutinin (WGA), Maclura pomifera agglutinin (MPA), Griffonia simplicifolia I agglutinin (GS I) and Bauhinea purpurea agglutinin (BPA). This was evidenced in the first three cases by a relative reversal of staining intensity between the acrosomal and post-acrosomal regions, and by a pronounced increase in the staining of the midpiece. Changes in the distribution of BPA binding sites were limited to the latter phenomenon. The results are compared with previous findings, discussed in the context of the hypermotility characteristic of ampullary sperm and related to previously reported differences in the lectin-binding patterns of the luminal and glandular epithelia.
对牛附睾尾和壶腹精子进行了目前的凝集素组织化学比较，以研究壶腹分泌物是否参与改变精子的质膜糖缀合物。基于以下异硫氰酸荧光素（FITC）标记的凝集素的结合模式变化，确实揭示了来自这两个区域的精子之间糖缀合物的显著重新分布：麦胚凝集素（WGA）、桑科榕属凝集素（MPA）、西非单叶豆凝集素I（GS I）和紫羊蹄甲凝集素（BPA）。在前三种情况下，这表现为顶体和顶体后区域之间染色强度的相对逆转，以及中段染色的明显增加。BPA结合位点分布的变化仅限于后一种现象。将结果与先前的发现进行比较，并在壶腹精子的高运动性特征的背景下进行讨论，并与先前报道的管腔和腺上皮的凝集素结合模式差异相关。
