Lin M H, Morshedi M, Srisombut C, Nassar A, Oehninger S
Department of Obstetrics and Gynecology, The Jones Insitute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk 23507, USA.
Fertil Steril. 1998 Dec;70(6):1148-55. doi: 10.1016/s0015-0282(98)00351-3.
[1] To examine the relationship between sperm membrane integrity and motion parameters before and after cryopreservation; [2] to determine the capacity of the membrane integrity tests to predict the outcome of cryopreservation in fertile and infertile men; and [3] to examine the degree of agreement between tail and head membrane integrity of testicular and ejaculated immotile sperm cryopreserved for intracytoplasmic sperm injection.
Prospective study.
Academic tertiary care institution.
PATIENT(S): Fertile donors and normozoospermic oligozoospermic, and asthenozoospermic subfertile men.
INTERVENTION(S): Semen samples were cryopreserved and thawed for analysis.
MAIN OUTCOME MEASURE(S): Sperm membrane integrity and computer-assisted motion parameters.
RESULT(S): The hypoosmotic swelling test and water test had a significant and positive correlation in the fresh and cryopreserved ejaculates of all groups. The results of the hypoosmotic swelling test correlated positively with the percent motility in the fresh ejaculates of fertile and subfertile men. None of the membrane integrity tests correlated with the cryosurvival rate in any group. In the ejaculated and testicular samples with no postcryopreservation motility, the simultaneous assessment of hypoosmotic swelling test and eosin showed that of 33% sperm exhibiting coiling with the hypoosmotic swelling test, only 9% were eosin negative, whereas 24% were eosin positive.
CONCLUSION(S): [1] The water test may be a simpler replacement for the hypoosmotic swelling test; [2] none of the membrane integrity tests predicted sperm motility after cryopreservation; and [3] there was a high degree of disagreement between the hypoosmotic swelling test and eosin in the samples with no postcryopreservation motility.
[1] 研究冷冻保存前后精子膜完整性与运动参数之间的关系；[2] 确定膜完整性检测预测生育男性和不育男性冷冻保存结果的能力；[3] 检测用于卵胞浆内单精子注射的冷冻保存的睾丸和射出的不动精子的尾部和头部膜完整性之间的一致程度。
前瞻性研究。
学术性三级医疗机构。
生育力正常的供者以及精子数量正常、少精子症和弱精子症的亚生育男性。
精液样本经冷冻保存后解冻用于分析。
精子膜完整性和计算机辅助运动参数。
在所有组的新鲜和冷冻保存的射出精液中，低渗肿胀试验和水试验具有显著正相关。低渗肿胀试验结果与生育男性和亚生育男性新鲜射出精液中的活动率呈正相关。在任何组中，没有一种膜完整性检测与冷冻存活率相关。在冷冻保存后无活动能力的射出和睾丸样本中，同时进行低渗肿胀试验和伊红染色评估显示，在低渗肿胀试验中表现出卷曲的精子中，只有9%伊红染色阴性，而24%伊红染色阳性。
[1] 水试验可能是低渗肿胀试验更简单的替代方法；[2] 没有一种膜完整性检测能预测冷冻保存后的精子活力；[3] 在冷冻保存后无活动能力的样本中，低渗肿胀试验和伊红染色之间存在高度不一致。
