Brown K E, Hibbs J R, Gallinella G, Anderson S M, Lehman E D, McCarthy P, Young N S
Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
N Engl J Med. 1994 Apr 28;330(17):1192-6. doi: 10.1056/NEJM199404283301704.
The presence of a specific cellular receptor is thought to be necessary for susceptibility to viral infection. The erythrocyte P antigen is the cellular receptor for parvovirus B19. We hypothesized that the rare persons with the p phenotype, whose erythrocytes do not have this receptor, would be naturally resistant to B19 infection, which causes erythema infectiosum.
Blood samples were collected from two populations in cross-sectional studies. We determined the P antigen phenotype of the red cells and tested plasma for anti-B19-specific antibodies. Bone marrow from donors of known P antigen phenotype was inoculated with parvovirus B19. Infectivity was measured by assays of erythroid progenitor cells, dot blot analysis, and in situ hybridization for B19 DNA, and an immunofluorescence assay for viral-capsid proteins.
Of the 17 subjects with the p red-cell phenotype, who did not have P antigen on their erythrocytes, none (0 of 11 and 0 of 6) had serologic evidence of previous parvovirus B19 infection. In contrast, the seropositivity rates in the two control groups were 71 percent (53 of 75, P < 0.001) and 47 percent (32 of 68, P = 0.03). In vitro, bone marrow from donors with the p phenotype maintained normal erythropoiesis despite very high concentrations of virus, with no evidence of infection of erythroid progenitor cells by parvovirus B19.
People who do not have P antigen, which is the cellular receptor for parvovirus B19, are naturally resistant to infection with this pathogen.
特定细胞受体的存在被认为是病毒感染易感性的必要条件。红细胞P抗原是细小病毒B19的细胞受体。我们推测，具有p表型的罕见个体，其红细胞没有这种受体，可能对导致传染性红斑的B19感染具有天然抵抗力。
在横断面研究中从两个人群采集血样。我们确定红细胞的P抗原表型，并检测血浆中抗B19特异性抗体。用细小病毒B19接种已知P抗原表型供体的骨髓。通过红系祖细胞测定、斑点印迹分析、B19 DNA原位杂交以及病毒衣壳蛋白免疫荧光测定来测量感染性。
在17名具有p红细胞表型（其红细胞上没有P抗原）的受试者中，没有人（11名中的0名和6名中的0名）有既往细小病毒B19感染的血清学证据。相比之下，两个对照组的血清阳性率分别为71%（75名中的53名，P<0.001）和47%（68名中的32名，P = 0.03）。在体外，尽管病毒浓度非常高，但具有p表型供体的骨髓仍维持正常的红细胞生成，没有证据表明细小病毒B19感染红系祖细胞。
没有作为细小病毒B19细胞受体的P抗原的人对该病原体感染具有天然抵抗力。
