Cusick M E
Department of Medical Biochemistry and Genetics, Texas A&M College of Medicine, College Station 77843-1114.
Nucleic Acids Res. 1994 Mar 11;22(5):869-77. doi: 10.1093/nar/22.5.869.
A previously unidentified ribonucleoprotein (RNP) gene of yeast has been cloned and sequenced. The gene, named RNP1, was found adjacent to a previously sequenced gene encoding the second gene for ribosomal protein L4. RNP1 contains two RNA Recognition Motifs (RRM), [alternatively known as RNA binding Domains (RBD)], but unlike most RNP genes does not contain any auxiliary simple sequence domains. The first RRM (RRM1) most resembles RRM domains found in the hnRNP A/B class of RNP proteins. The second RRM (RRM2) most resembles a RRM so far seen only in the single RRM of the yeast SSB1 gene. Two null mutants of RNP1 that were created, a frameshift disruption and a complete deletion of the gene, were viable, demonstrating that the gene is not essential for cell growth. Two double null mutants of yeast RNP genes that were created (delta RNP1/delta SSB1 and delta SSB1/delta NPL3) were also viable. A fragment identical in size to the RRM1 domain could be amplified by PCR from the DNA of fungi, plants, and animals, using primers matching the ends of this domain, indicating that the structure of RRM1 is conserved. Another potential open reading frame on the same cloned fragment of DNA encodes a gene product whose structure resembles that of a seven-transmembrane-segment membrane receptor protein.
酵母中一个先前未被鉴定的核糖核蛋白（RNP）基因已被克隆并测序。该基因名为RNP1，位于一个先前已测序的基因附近，该基因编码核糖体蛋白L4的第二个基因。RNP1包含两个RNA识别基序（RRM），[也称为RNA结合结构域（RBD）]，但与大多数RNP基因不同的是，它不包含任何辅助简单序列结构域。第一个RRM（RRM1）与hnRNP A/B类RNP蛋白中的RRM结构域最为相似。第二个RRM（RRM2）与迄今为止仅在酵母SSB1基因的单个RRM中发现的RRM最为相似。构建的两个RNP1无效突变体，一个是移码破坏突变体，另一个是基因完全缺失突变体，都是有活力的，这表明该基因对细胞生长不是必需的。构建的两个酵母RNP基因双无效突变体（delta RNP1/delta SSB1和delta SSB1/delta NPL3）也有活力。使用与该结构域末端匹配的引物，通过PCR可以从真菌、植物和动物的DNA中扩增出与RRM1结构域大小相同的片段，这表明RRM1的结构是保守的。在同一克隆的DNA片段上的另一个潜在开放阅读框编码一种基因产物，其结构类似于七跨膜段膜受体蛋白。
