酿酒酵母六条最小染色体的物理图谱，分辨率为2.6千碱基对。
Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs.
作者信息
Riles L, Dutchik J E, Baktha A, McCauley B K, Thayer E C, Leckie M P, Braden V V, Depke J E, Olson M V
机构信息
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.
出版信息
Genetics. 1993 May;134(1):81-150. doi: 10.1093/genetics/134.1.81.
Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae are presented. In order of increasing size, they are chromosomes I, VI, III, IX, V and VIII, comprising 2.49 megabase pairs of DNA. The maps are based on the analysis of an overlapping set of lambda and cosmid clones. Overlaps between adjacent clones were recognized by shared restriction fragments produced by the combined action of EcoRI and HindIII. The average spacing between mapped cleavage sites is 2.6 kb. Five of the six chromosomes were mapped from end to end without discontinuities; a single internal gap remains in the map of chromosome IX. The reported maps span an estimated 97% of the DNA on the six chromosomes; nearly all the missing segments are telomeric. The maps are fully cross-correlated with the previously published SfiI/NotI map of the yeast genome by A. J. Link and M. V. Olson. They have also been cross-correlated with the yeast genetic map at 51 loci.
展示了酿酒酵母六条最小染色体的物理图谱。按大小递增顺序，它们分别是I、VI、III、IX、V和VIII号染色体，包含249万个碱基对的DNA。这些图谱基于对一组重叠的λ噬菌体和黏粒克隆的分析。相邻克隆之间的重叠通过EcoRI和HindIII联合作用产生的共享限制片段来识别。定位的切割位点之间的平均间距为2.6 kb。六条染色体中的五条从头到尾都已定位，没有间断；IX号染色体的图谱中仍有一个单一的内部缺口。所报道的图谱涵盖了六条染色体上估计97%的DNA；几乎所有缺失的片段都是端粒。这些图谱与A. J. Link和M. V. Olson之前发表的酵母基因组SfiI/NotI图谱完全相互关联。它们还与酵母遗传图谱在51个位点上进行了相互关联。
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