Godin D V, Cheng K M, Garnett M E, Nichols C R
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver.
Can J Cardiol. 1994 Mar;10(2):221-8.
To compare levels of antioxidant components of blood and aortic tissue from two strains of Japanese quail showing high and low susceptibilities to atherosclerosis and receiving either control or cholesterol-enriched diets.
All experimental birds were placed on the control diet until eight weeks of age. Twenty males of each strain were then placed on the atherogenic diet (control diet supplemented with 1% cholesterol) and another 20 males of each strain were continued on the control diet for an additional 10 weeks.
In the absence of cholesterol supplementation, plasma cholesterol and triglyceride and antioxidant enzyme profiles of aorta, red blood cells and plasma were comparable in the two groups of birds, with the exception of a small, but significant (P < 0.05), decrease in aortic glutathione reductase in susceptible animals (0.378 nmol/min/mg) relative to the resistant birds (0.441 nmol/min/mg). Cholesterol feeding produced larger increases in cholesterol and triglycerides and more extensive atherosclerotic plaque formation in susceptible compared with resistant quail. In the susceptible group, significant negative correlations were noted between severity of atherosclerotic lesions and aortic superoxide dismutase (correlation coefficient -0.752) and glutathione reductase (correlation coefficient -0.775) activities. Cholesterol feeding was associated with increased activity of plasma glutathione peroxidase and superoxide dismutase activities in both strains, although only the susceptible birds showed significant positive correlations of superoxide dismutase activities with plasma cholesterol (correlation coefficient +0.694) and triglycerides (correlation coefficient +0.669). Peroxide-induced depletion of glutathione in red blood cells was unaffected by cholesterol feeding, although red blood cell susceptibility to lipid peroxidation was decreased to an equivalent degree in both strains.
Susceptible and resistant quail are biochemically distinct in terms of alterations in antioxidant components produced by dietary cholesterol supplementation. Results of the present study are consistent with the proposed involvement of oxidative processes in the pathogenesis of atherosclerosis.
比较两种对动脉粥样硬化易感性高低不同且分别接受对照饮食或高胆固醇饮食的日本鹌鹑品系的血液和主动脉组织中抗氧化成分的水平。
所有实验鸟类在8周龄前均喂食对照饮食。然后，每个品系的20只雄性鹌鹑改为喂食致动脉粥样硬化饮食（对照饮食添加1%胆固醇），每个品系的另外20只雄性鹌鹑继续喂食对照饮食，持续10周。
在不补充胆固醇的情况下，两组鸟类的血浆胆固醇、甘油三酯以及主动脉、红细胞和血浆中的抗氧化酶谱相当，但易感动物（0.378纳摩尔/分钟/毫克）的主动脉谷胱甘肽还原酶相对于抗性鸟类（0.441纳摩尔/分钟/毫克）有小幅但显著（P<0.05）下降。与抗性鹌鹑相比，喂食胆固醇后，易感鹌鹑的胆固醇和甘油三酯升高幅度更大，动脉粥样硬化斑块形成更广泛。在易感组中，动脉粥样硬化病变严重程度与主动脉超氧化物歧化酶（相关系数-0.752）和谷胱甘肽还原酶（相关系数-0.775）活性之间存在显著负相关。喂食胆固醇后，两个品系的血浆谷胱甘肽过氧化物酶活性和超氧化物歧化酶活性均增加，不过只有易感鸟类的超氧化物歧化酶活性与血浆胆固醇（相关系数+0.694）和甘油三酯（相关系数+0.669）呈显著正相关。尽管两个品系的红细胞对脂质过氧化的敏感性均同等程度降低，但胆固醇喂养并未影响过氧化物诱导的红细胞谷胱甘肽消耗。
在饮食补充胆固醇所产生的抗氧化成分变化方面，易感和抗性鹌鹑在生化特性上存在差异。本研究结果与氧化过程参与动脉粥样硬化发病机制的观点一致。
