• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

胆固醇代谢和转运相关的七个基因在易患和不易患动脉粥样硬化的日本鹌鹑品系肝脏中的差异 mRNA 表达。

Differential mRNA expression of seven genes involved in cholesterol metabolism and transport in the liver of atherosclerosis-susceptible and -resistant Japanese quail strains.

机构信息

Avian Research Centre, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, Canada.

出版信息

Genet Sel Evol. 2012 Jun 8;44(1):20. doi: 10.1186/1297-9686-44-20.

DOI:10.1186/1297-9686-44-20
PMID:22682430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3430562/
Abstract

BACKGROUND

Two atherosclerosis-susceptible and -resistant Japanese quail (Coturnix japonica) strains obtained by divergent selection are commonly used as models to study atherosclerosis, but no genetic characterization of their phenotypic differences has been reported so far. Our objective was to examine possible differences in the expression of genes involved in cholesterol metabolism and transport in the liver between these two strains and to evaluate the value of this model to analyze the gene system affecting cholesterol metabolism and transport.

METHODS

A factorial study with both strains (atherosclerosis-susceptible versus atherosclerosis-resistant) and two diets (control versus cholesterol) was carried out. The mRNA concentrations of four genes involved in cholesterol biosynthesis (HMGCR, FDFT1, SQLE and DHCR7) and three genes in cholesterol transport (ABCG5, ABCG8 and APOA1) were assayed using real-time quantitative PCR. Plasma lipids were also assayed.

RESULTS

Expression of ABCG5 (control diet) and ABCG8 (regardless of dietary treatment) and expression of HMGCR, FDFT1 and SQLE (regardless of dietary treatment) were significantly higher in the atherosclerosis-resistant than in the atherosclerosis-susceptible strain. Plasma triglyceride and LDL levels, and LDL/HDL ratio were significantly higher in the atherosclerosis-susceptible than in the atherosclerosis-resistant strain fed the cholesterol diet. In the atherosclerosis-susceptible strain, ABCG5 expression regressed significantly and positively on plasma LDL level, whereas DHCR7 and SQLE expression regressed significantly and negatively on plasma triglyceride level.

CONCLUSIONS

Our results provide support for the hypothesis that the atherosclerosis-resistant strain metabolizes and excretes cholesterol faster than the atherosclerosis-susceptible strain. We have also demonstrated that these quail strains are a useful model to study cholesterol metabolism and transport in relation with atherosclerosis.

摘要

背景

两种通过分歧选择获得的易患和抗动脉粥样硬化的日本鹌鹑（Coturnix japonica）品系常用于动脉粥样硬化的研究模型，但迄今为止尚未报道其表型差异的遗传特征。我们的目的是研究这两个品系之间肝脏中胆固醇代谢和转运相关基因表达的可能差异，并评估该模型分析影响胆固醇代谢和转运的基因系统的价值。

方法

采用因子设计，在两个品系（易患动脉粥样硬化与抗动脉粥样硬化）和两种饮食（对照与胆固醇）之间进行研究。使用实时定量 PCR 测定了胆固醇生物合成的四个基因（HMGCR、FDFT1、SQLE 和 DHCR7）和胆固醇转运的三个基因（ABCG5、ABCG8 和 APOA1）的 mRNA 浓度。还测定了血浆脂质。

结果

在抗动脉粥样硬化品系中，ABCG5（对照饮食）和 ABCG8（无论饮食处理如何）以及 HMGCR、FDFT1 和 SQLE（无论饮食处理如何）的表达显著高于易患动脉粥样硬化品系。在喂胆固醇饮食的易患动脉粥样硬化品系中，血浆甘油三酯和 LDL 水平以及 LDL/HDL 比值显著高于抗动脉粥样硬化品系。在易患动脉粥样硬化品系中，ABCG5 的表达与血浆 LDL 水平呈显著正相关，而 DHCR7 和 SQLE 的表达与血浆甘油三酯水平呈显著负相关。

结论

我们的结果支持这样一种假设，即抗动脉粥样硬化品系比易患动脉粥样硬化品系更快地代谢和排泄胆固醇。我们还证明，这些鹌鹑品系是研究与动脉粥样硬化相关的胆固醇代谢和转运的有用模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/f556bf089c75/1297-9686-44-20-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/a789e05aa187/1297-9686-44-20-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/d0f8b975b29e/1297-9686-44-20-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/11153484b1fb/1297-9686-44-20-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/10b42c7f7aed/1297-9686-44-20-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/340e6cd4b705/1297-9686-44-20-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/f556bf089c75/1297-9686-44-20-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/a789e05aa187/1297-9686-44-20-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/d0f8b975b29e/1297-9686-44-20-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/11153484b1fb/1297-9686-44-20-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/10b42c7f7aed/1297-9686-44-20-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/340e6cd4b705/1297-9686-44-20-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a585/3430562/f556bf089c75/1297-9686-44-20-6.jpg

相似文献

1
Differential mRNA expression of seven genes involved in cholesterol metabolism and transport in the liver of atherosclerosis-susceptible and -resistant Japanese quail strains.胆固醇代谢和转运相关的七个基因在易患和不易患动脉粥样硬化的日本鹌鹑品系肝脏中的差异 mRNA 表达。
Genet Sel Evol. 2012 Jun 8;44(1):20. doi: 10.1186/1297-9686-44-20.
2
Effect of cholesterol feeding on serum lipoproteins and atherosclerosis in atherosclerosis-susceptible and atherosclerosis-resistant Japanese quail.胆固醇喂养对易患动脉粥样硬化和抗动脉粥样硬化日本鹌鹑血清脂蛋白及动脉粥样硬化的影响。
Poult Sci. 1982 Dec;61(12):2407-14. doi: 10.3382/ps.0612407.
3
Antioxidant status of Japanese quail: comparison of atherosclerosis-susceptible and -resistant strains.日本鹌鹑的抗氧化状态：动脉粥样硬化易感和抗性品系的比较。
Can J Cardiol. 1994 Mar;10(2):221-8.
4
Dietary induction of hypercholesterolemia and atherosclerosis in Japanese quail of strain SEA.SEA品系日本鹌鹑高胆固醇血症和动脉粥样硬化的饮食诱导
J Nutr. 1985 Sep;115(9):1154-61. doi: 10.1093/jn/115.9.1154.
5
Effect of dietary cholesterol on the activities of key enzymes of cholesterol metabolism in hyperlipidemia- and atherosclerosis-prone Japanese quail.膳食胆固醇对易患高脂血症和动脉粥样硬化的日本鹌鹑胆固醇代谢关键酶活性的影响。
J Nutr Sci Vitaminol (Tokyo). 1996 Aug;42(4):287-300. doi: 10.3177/jnsv.42.287.
6
Influence of dietary lipids on hepatic mRNA levels of proteins regulating plasma lipoproteins in baboons with high and low levels of large high density lipoproteins.饮食脂质对大高密度脂蛋白水平高低不同的狒狒中调节血浆脂蛋白的蛋白质肝脏mRNA水平的影响。
J Lipid Res. 1991 Dec;32(12):1929-40.
7
Interactive effects of increased intake of saturated fat and cholesterol on atherosclerosis in the Japanese quail (Coturnix japonica).饱和脂肪和胆固醇摄入量增加对日本鹌鹑（Coturnix japonica）动脉粥样硬化的交互作用。
Br J Nutr. 1998 Jul;80(1):89-100. doi: 10.1017/s0007114598001810.
8
Effect of hempseed (Cannabis sativa L.) on performance, egg traits and blood biochemical parameters and antioxidant activity in laying Japanese Quail (Coturnix coturnix japonica).大麻籽（Cannabis sativa L.）对日本鹌鹑（Coturnix coturnix japonica）产蛋性能、蛋品质、血液生化参数及抗氧化活性的影响
Br Poult Sci. 2014;55(6):785-94. doi: 10.1080/00071668.2014.978264.
9
Diet composition and cholesteremia in Japanese quail.日本鹌鹑的饮食组成与胆固醇血症
Poult Sci. 1977 Nov;56(6):2108-10. doi: 10.3382/ps.0562108.
10
Transcriptome analysis of genes regulated by cholesterol loading in two strains of mouse macrophages associates lysosome pathway and ER stress response with atherosclerosis susceptibility.胆固醇负荷调节两种品系小鼠巨噬细胞基因表达谱分析与动脉粥样硬化易感性的关系。溶酶体途径和内质网应激反应与动脉粥样硬化易感性相关。
PLoS One. 2013 May 21;8(5):e65003. doi: 10.1371/journal.pone.0065003. Print 2013.

引用本文的文献

1
Integrated transcriptomic and proteomic analyses reveal the mechanism of easy acceptance of artificial pelleted diets during food habit domestication in Largemouth bass (Micropterus salmoides).整合转录组和蛋白质组分析揭示大口黑鲈食性驯化过程中易于接受人工颗粒饲料的机制。
Sci Rep. 2023 Oct 27;13(1):18461. doi: 10.1038/s41598-023-45645-8.
2
RNA Sequencing-Based Whole-Transcriptome Analysis of Friesian Cattle Fed with Grape Pomace-Supplemented Diet.基于RNA测序的饲喂添加葡萄皮渣日粮的弗里斯兰奶牛全转录组分析。
Animals (Basel). 2018 Oct 23;8(11):188. doi: 10.3390/ani8110188.
3
Interaction of genotype and diet on small intestine microbiota of Japanese quail fed a cholesterol enriched diet.

本文引用的文献

1
ABCG5/G8 polymorphisms and markers of cholesterol metabolism: systematic review and meta-analysis.ABCG5/G8 多态性与胆固醇代谢标志物：系统评价和荟萃分析。
J Lipid Res. 2010 Oct;51(10):3016-23. doi: 10.1194/jlr.M008128. Epub 2010 Jun 25.
2
Biological role of liver X receptors.肝脏X受体的生物学作用。
J Physiol Pharmacol. 2008 Dec;59 Suppl 7:31-55.
3
Laboratory evaluation of a novel capillary blood sampling device for measuring eight clinical chemistry parameters and HbA1c.一种用于测量八项临床化学参数和糖化血红蛋白（HbA1c）的新型毛细血管采血装置的实验室评估。
富含胆固醇饮食对日本鹌鹑小肠微生物群的基因型和饮食相互作用。
Sci Rep. 2018 Feb 5;8(1):2381. doi: 10.1038/s41598-018-20508-9.
4
Prediction and analysis of essential genes using the enrichments of gene ontology and KEGG pathways.利用基因本体论和KEGG通路的富集对必需基因进行预测和分析。
PLoS One. 2017 Sep 5;12(9):e0184129. doi: 10.1371/journal.pone.0184129. eCollection 2017.
5
SIRT1/Atg5/autophagy are involved in the antiatherosclerosis effects of ursolic acid.沉默调节蛋白1/自噬相关蛋白5/自噬参与了熊果酸的抗动脉粥样硬化作用。
Mol Cell Biochem. 2016 Sep;420(1-2):171-84. doi: 10.1007/s11010-016-2787-x. Epub 2016 Aug 11.
6
How Research on Human Progeroid and Antigeroid Syndromes Can Contribute to the Longevity Dividend Initiative.关于人类早衰症和抗衰老综合征的研究如何为长寿红利计划做出贡献。
Cold Spring Harb Perspect Med. 2016 Apr 1;6(4):a025882. doi: 10.1101/cshperspect.a025882.
7
The effect of diet and host genotype on ceca microbiota of Japanese quail fed a cholesterol enriched diet.饮食和宿主基因型对饲喂富含胆固醇日粮的日本鹌鹑盲肠微生物群的影响。
Front Microbiol. 2015 Oct 7;6:1092. doi: 10.3389/fmicb.2015.01092. eCollection 2015.
8
Integromic analysis of genetic variation and gene expression identifies networks for cardiovascular disease phenotypes.基因变异与基因表达的整合分析确定了心血管疾病表型的网络。
Circulation. 2015 Feb 10;131(6):536-49. doi: 10.1161/CIRCULATIONAHA.114.010696. Epub 2014 Dec 22.
Clin Chim Acta. 2009 Mar;401(1-2):152-7. doi: 10.1016/j.cca.2008.12.016. Epub 2008 Dec 24.
4
Cholesterol feedback: from Schoenheimer's bottle to Scap's MELADL.胆固醇反馈：从舍恩海默的瓶子到斯卡普的MELADL
J Lipid Res. 2009 Apr;50 Suppl(Suppl):S15-27. doi: 10.1194/jlr.R800054-JLR200. Epub 2008 Oct 29.
5
Both beta-actin and GAPDH are useful reference genes for normalization of quantitative RT-PCR in human FFPE tissue samples of prostate cancer.β-肌动蛋白和甘油醛-3-磷酸脱氢酶都是用于前列腺癌人类甲醛固定石蜡包埋（FFPE）组织样本定量逆转录聚合酶链反应（RT-PCR）标准化的有用内参基因。
Prostate. 2008 Oct 1;68(14):1555-60. doi: 10.1002/pros.20815.
6
Differential expression of hepatic genes involved in cholesterol homeostasis in high- and low-responding strains of laboratory opossums.实验负鼠高反应和低反应品系中参与胆固醇稳态的肝脏基因的差异表达
Metabolism. 2008 May;57(5):718-24. doi: 10.1016/j.metabol.2008.01.018.
7
Rapid transcriptome characterization for a nonmodel organism using 454 pyrosequencing.使用454焦磷酸测序技术对非模式生物进行快速转录组特征分析。
Mol Ecol. 2008 Apr;17(7):1636-47. doi: 10.1111/j.1365-294X.2008.03666.x. Epub 2008 Feb 5.
8
ATP-Binding cassette cholesterol transporters and cardiovascular disease.ATP结合盒式胆固醇转运蛋白与心血管疾病
Circ Res. 2006 Nov 10;99(10):1031-43. doi: 10.1161/01.RES.0000250171.54048.5c.
9
High-density lipoprotein function recent advances.高密度脂蛋白功能的最新进展。
J Am Coll Cardiol. 2005 Nov 15;46(10):1792-8. doi: 10.1016/j.jacc.2005.06.080. Epub 2005 Oct 19.
10
Human apolipoprotein A-I and A-I mimetic peptides: potential for atherosclerosis reversal.人载脂蛋白A-I与A-I模拟肽：逆转动脉粥样硬化的潜力。
Curr Opin Lipidol. 2004 Dec;15(6):645-9. doi: 10.1097/00041433-200412000-00004.