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牛疱疹病毒1型猪分离株的限制性内切酶分析

Restriction endonuclease analysis of a porcine isolate of bovine herpesvirus type 1.

作者信息

Várady E, Tuboly T, Derbyshire J B

机构信息

Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph.

出版信息

Can J Vet Res. 1994 Jan;58(1):65-6.

Abstract

Deoxyribonucleic acid (DNA) was extracted from bovine herpesvirus type 1 (BHV-1) isolated from a stillborn porcine fetus, from the Cooper reference strain of BHV-1, and from an Ontario bovine respiratory isolate. Each DNA was digested with the restriction endonucleases HindIII, EcoRI, HpaI and BamHI. Except for very minor differences in the patterns produced after digestion with EcoRI and HindIII, the DNA of the porcine isolate reacted in a similar manner to the bovine viruses, and it was concluded that the porcine virus is genetically similar to bovine isolates of BHV-1.

摘要

从一头死产猪胎儿中分离出的1型牛疱疹病毒(BHV-1)、BHV-1的库珀参考毒株以及一株安大略牛呼吸道分离株中提取了脱氧核糖核酸(DNA)。每种DNA都用限制性内切酶HindIII、EcoRI、HpaI和BamHI进行消化。除了用EcoRI和HindIII消化后产生的图谱有非常细微的差异外,猪分离株的DNA与牛病毒的反应方式相似,由此得出结论,该猪病毒在基因上与BHV-1的牛分离株相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fdd/1263661/23992f39d431/cjvetres00029-0069-a.jpg

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