Joo H S, Dee S A, Molitor T W, Thacker B J
Am J Vet Res. 1984 Oct;45(10):1924-7.
The pathogenesis of infectious bovine rhinotracheitis (IBR) virus (bovine herpesvirus-1) was studied in porcine fetuses after in utero inoculation. Laparotomies were performed on 8 seronegative pregnant sows at 34 to 86 days of gestation, and all fetuses in 1 uterine horn of each sow were exposed to IBR virus via inoculation into the amniotic sacs. Fetuses in the other horn served as controls. Clinical signs of infection were not observed in the sows, except for 2 sows that aborted at postinoculation days (PID) 11 and 15. Fetuses of the remaining 6 sows were collected at slaughter on PID 15 to 28. Fetuses were examined for gross abnormalities, presence of IBR virus in tissues, and the formation of neutralizing antibodies to IBR virus. Of 33 inoculated fetuses from 6 sows, 10 were mummified, 11 were hemorrhagic and/or edematous, and 12 were alive. Necrotic lesions were observed on the skin and in the liver of dead and live fetuses. Virus was recovered from 29 of 33 inoculated fetuses. Infectious bovine rhinotracheitis virus was isolated from fetal skin, liver, lungs, kidney, spleen, stomach contents, brain, amniotic fluid, and placenta. Virus was isolated from 4 of 11 fetuses recovered from 1 aborting sow. Antibodies to IBR virus were not detected in sera from the sows. However, antibodies were detected in 6 of 15 fetuses inoculated at 63 to 86 days of gestation and collected at slaughter at 86 to 112 days of gestation. The youngest fetus with detectable IBR antibody was estimated to be 74 days of gestation by measuring crown-rump length of the fetus.
在子宫内接种后，对猪胎儿感染牛传染性鼻气管炎（IBR）病毒（牛疱疹病毒1型）的发病机制进行了研究。在妊娠34至86天对8头血清阴性的怀孕母猪进行剖腹手术，每头母猪一个子宫角内的所有胎儿通过接种羊膜囊暴露于IBR病毒。另一个子宫角内的胎儿作为对照。除2头在接种后第11天和第15天流产的母猪外，未观察到其他母猪有感染的临床症状。其余6头母猪的胎儿在接种后第15至28天屠宰时收集。检查胎儿有无肉眼可见的异常、组织中IBR病毒的存在以及针对IBR病毒的中和抗体的形成。在来自6头母猪的33头接种胎儿中，10头为干尸化，11头有出血和/或水肿，12头存活。在死亡和存活胎儿的皮肤和肝脏中观察到坏死病变。从33头接种胎儿中的29头中分离出病毒。从胎儿皮肤、肝脏、肺、肾、脾、胃内容物、脑、羊水和胎盘中分离出牛传染性鼻气管炎病毒。从1头流产母猪的11头存活胎儿中的4头中分离出病毒。在母猪血清中未检测到针对IBR病毒的抗体。然而，在妊娠63至86天接种、妊娠86至112天屠宰时收集的15头胎儿中的6头检测到了抗体。通过测量胎儿的顶臀长度，估计最早检测到IBR抗体的胎儿为妊娠74天。
