Ronquist G, Wedenberg K, Waldenström A, Ericson A, Ulmsten U
Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
Clin Chim Acta. 1993 Dec 31;223(1-2):93-102. doi: 10.1016/0009-8981(93)90065-c.
We determined the concentrations of adenosine and some of its catabolic products in biopsy specimens from predetermined loci of human myometrium under different functional conditions to compare uterine muscle with rectus abdominis muscle from the same individuals. In order to achieve a good resolution in the separation of nucleosides and purine bases, a preseparation procedure was developed prior to analysis of these compounds on high performance liquid chromatography. Adenosine occurred in a nearly 70-fold higher concentration in smooth uterine muscle in comparison with striated skeletal muscle. Similarly, myometrial inosine and hypoxanthine were 7- and 2.4-times in excess over the rectus muscle, whereas xanthine was scarcely and rather evenly represented in the two types of muscles. The uterine content of adenosine and inosine was distinctly higher in pregnant women compared to non-pregnant ones. A regional difference existed for adenosine, with 3.3 times higher concentration in fundus uteri compared to the isthmic part. A reverse pattern was observed for hypoxanthine and inosine, being 2-3 times more frequent in the isthmic part. The orthophosphate concentration was not stoichiometrically related to the adenosine concentration in a simple way, being 2-3 times lower in uterine muscle compared to the skeletal muscle. A significant correlation existed between uterine contents of AMP and adenosine and similarly, significant inverse correlations were apparent between uterine ATP and ADP contents and energy charge on one hand and adenosine content on the other.
我们测定了在不同功能状态下人体子宫肌层预定部位活检标本中腺苷及其一些分解代谢产物的浓度，以便将子宫肌与同一受试者的腹直肌进行比较。为了在核苷和嘌呤碱的分离中获得良好的分辨率，在对这些化合物进行高效液相色谱分析之前，开发了一种预分离程序。与横纹肌相比，腺苷在子宫平滑肌中的浓度高出近70倍。同样，子宫肌层中的肌苷和次黄嘌呤比腹直肌分别高出7倍和2.4倍，而黄嘌呤在这两种肌肉中的含量很少且分布较为均匀。与未怀孕的女性相比，孕妇子宫中腺苷和肌苷的含量明显更高。腺苷存在区域差异，子宫底部的浓度比峡部高3.3倍。次黄嘌呤和肌苷则呈现相反的模式，在峡部的含量高出2 - 3倍。正磷酸盐浓度与腺苷浓度之间不存在简单的化学计量关系，子宫肌中的浓度比骨骼肌低2 - 3倍。子宫中AMP和腺苷的含量之间存在显著相关性，同样，子宫ATP和ADP含量以及能量电荷与腺苷含量之间一方面存在显著的负相关。
