Ingram R T, Bonde S K, Riggs B L, Fitzpatrick L A
Department of Medicine, Mayo Clinic, Rochester, MN 55905.
Differentiation. 1994 Jan;55(2):153-63. doi: 10.1046/j.1432-0436.1994.5520153.x.
Individually, transforming growth factor beta (TGF beta) and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) alter the growth and differentiation of normal and transformed osteoblast-like (OB) cells. Although recent evidence suggests interactions between TGF beta and 1,25(OH)2D3 may occur, little is known of the individual or combined effects of these hormones on the expression of the osteoblast phenotype at the cytochemical and biochemical levels in normal human OB (hOB) cells. Primary cultures of hOBs were treated with TGF beta (0.001-10 ng/ml) and 1,25(OH)2D3 (0.1 pM-100 nM) either alone or in combination. TGF beta and 1,25(OH)2D3 stimulated spindle-shaped cells to become stellate in appearance and increased the number of cytoplasmic processes. TGF beta increased 3H-thymidine incorporation and 1,25(OH)2D3 reduced this effect. Conversely, procollagen type-I synthesis and secretion were increased in a dose-dependent manner in the presence of TGF beta but were not significantly affected in the presence of 1,25(OH)2D3. TGF beta and 1,25(OH)2D3 each marginally increased alkaline phosphatase (ALP) activity, but the combination synergistically increased ALP activity in a dose- and time-dependent manner at the cytochemical and biochemical level (three to tenfold over vehicle controls; n = 12). In contrast, TGF beta reduced 1,25(OH)2D3-stimulated osteocalcin secretion. These data suggest that TGF beta stimulates hOB cells to actively produce collagen matrix and proliferate. The combination of TGF beta and 1,25(OH)2D3, however, produces a synergistic increase in ALP activity and maintenance of collagen synthesis. 1,25(OH)2D3 stimulation may induce cells to advance to an endstage where cell proliferation is reduced and osteocalcin expression is promoted. Interactions between TGF beta and 1,25(OH)2D3 may represent important steps in the regulation of osteoblast differentiation and matrix production.
单独来看，转化生长因子β（TGFβ）和1,25 - 二羟基维生素D3（1,25(OH)2D3）会改变正常及转化的成骨样（OB）细胞的生长和分化。尽管最近的证据表明TGFβ与1,25(OH)2D3之间可能存在相互作用，但对于这些激素在正常人成骨细胞（hOB）细胞的细胞化学和生化水平上对成骨细胞表型表达的单独或联合作用，人们了解甚少。将hOB的原代培养物分别用TGFβ（0.001 - 10 ng/ml）和1,25(OH)2D3（0.1 pM - 100 nM）单独处理或联合处理。TGFβ和1,25(OH)2D3刺激梭形细胞外观变为星状，并增加了细胞质突起的数量。TGFβ增加了3H - 胸腺嘧啶核苷掺入量，而1,25(OH)2D3降低了这种作用。相反，在TGFβ存在下，I型前胶原的合成和分泌呈剂量依赖性增加，但在1,25(OH)2D3存在下没有受到显著影响。TGFβ和1,25(OH)2D3各自略微增加了碱性磷酸酶（ALP）活性，但在细胞化学和生化水平上，二者联合以剂量和时间依赖性方式协同增加了ALP活性（比溶剂对照高3至10倍；n = 12）。相比之下，TGFβ降低了1,25(OH)2D3刺激的骨钙素分泌。这些数据表明，TGFβ刺激hOB细胞积极产生胶原基质并增殖。然而，TGFβ和
