Bindra A, Khuller G K
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh.
Indian J Biochem Biophys. 1993 Oct;30(5):311-3.
The influence of intracellular cAMP on phosphatidylcholine biosynthesis in Microsporum gypseum has been examined using radiolabelled precursors. The incorporation of labelled choline, methionine and ethanolamine into total lipids, phospholipids and choline containing phospholipids increased in aminophylline and decreased in atropine grown cells as a result of rise and fall in cAMP levels in these cells. The enhanced uptake of labelled methionine and ethanolamine in comparison to labelled choline in choline containing phospholipids in aminophylline grown cells suggests that methylation pathway is more influenced by cAMP than CDP-choline pathway.
利用放射性标记前体研究了细胞内cAMP对石膏样小孢子菌中磷脂酰胆碱生物合成的影响。由于这些细胞中cAMP水平的升高和降低，在氨茶碱中生长的细胞中，标记的胆碱、蛋氨酸和乙醇胺掺入总脂质、磷脂和含胆碱磷脂的量增加，而在阿托品中生长的细胞中则减少。与氨茶碱中生长的细胞中含胆碱磷脂中标记的胆碱相比，标记的蛋氨酸和乙醇胺摄取增加，这表明甲基化途径比CDP-胆碱途径受cAMP的影响更大。
