Bindra A, Khuller G K
Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh, India.
Indian J Biochem Biophys. 1993 Feb;30(1):71-2.
Aminophylline added to the growth medium of Microsporum gypseum for varying periods exhibited varied effects on lipid synthesis. A decreased incorporation of [14C]acetate into both lipids and phospholipids was initially observed which showed increase after 24 hr of incubation. Short-time exposure of aminophylline also resulted in decreased activity of glycerol-3-phosphate acyltransferase, the key enzyme of lipid synthesis, which, however, got stimulated on longer incubation, supporting the decreased/increased synthesis of lipids during short/long time exposure. Changes in the intracellular levels of cAMP at different time points account for the induced alterations in lipid synthesis, possibly due to formation of metabolites of aminophylline during growth of the organism.
在不同时间段向石膏样小孢子菌的生长培养基中添加氨茶碱，对脂质合成表现出不同的影响。最初观察到[14C]乙酸盐掺入脂质和磷脂的量减少，孵育24小时后显示增加。氨茶碱的短期暴露还导致脂质合成的关键酶甘油-3-磷酸酰基转移酶的活性降低，然而，长时间孵育后该酶受到刺激，这支持了在短时间/长时间暴露期间脂质合成的减少/增加。不同时间点细胞内cAMP水平的变化解释了脂质合成中诱导的改变，这可能是由于生物体生长过程中氨茶碱代谢产物的形成。
