一种杂合芳香环双加氧酶对三氯乙烯的高效降解
Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase.
作者信息
Furukawa K, Hirose J, Hayashida S, Nakamura K
机构信息
Department of Agricultural Chemistry, Kyushu University, Fukuoka, Japan.
出版信息
J Bacteriol. 1994 Apr;176(7):2121-3. doi: 10.1128/jb.176.7.2121-2123.1994.
Abstract
Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of biphenyl terminal dioxygenase in Pseudomonas pseudoalcaligenes KF707), bphA3 (the gene encoding ferredoxin in KF707), and bphA4 (the gene encoding ferredoxin reductase in KF707) degraded trichloroethylene much faster than E. coli cells carrying the original toluene dioxygenase genes (todC1C2BA) or the original biphenyl dioxygenase genes (bphA1A2A3A4).
摘要
甲苯代谢tod操纵子和联苯代谢bph操纵子之间的杂交基因簇工程极大地提高了三氯乙烯的生物降解速率。携带由todC1（编码恶臭假单胞菌F1中甲苯末端双加氧酶大亚基的基因）、bphA2（编码假产碱假单胞菌KF707中联苯末端双加氧酶小亚基的基因）、bphA3（编码KF707中铁氧还蛋白的基因）和bphA4（编码KF707中铁氧还蛋白还原酶的基因）组成的杂交基因簇的大肠杆菌细胞降解三氯乙烯的速度比携带原始甲苯双加氧酶基因（todC1C2BA）或原始联苯双加氧酶基因（bphA1A2A3A4）的大肠杆菌细胞快得多。
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