含有克隆的恶臭假单胞菌F1甲苯双加氧酶基因的大肠杆菌对三氯乙烯的降解作用
Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.
作者信息
Zylstra G J, Wackett L P, Gibson D T
机构信息
Department of Microbiology, University of Iowa, Iowa City 52242.
出版信息
Appl Environ Microbiol. 1989 Dec;55(12):3162-6. doi: 10.1128/aem.55.12.3162-3166.1989.
Abstract
Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroethylene degradation by the recombinant organism depended on the cell concentration and the concentration of trichloroethylene. A linear rate of trichloroethylene degradation was observed with the E. coli recombinant strain. In contrast, P. putida F39/D, a mutant strain of P. putida F1 that does not contain cis-toluene dihydrodiol dehydrogenase, showed a much faster initial rate of trichloroethylene degradation which decreased over time.
摘要
恶臭假单胞菌F1中的甲苯双加氧酶被认为是一种能够降解三氯乙烯的酶。现在，含有在tac启动子控制下甲苯双加氧酶结构基因（todC1C2BA）的大肠杆菌JM109(pDTG601)已证实了这一点。重组生物对三氯乙烯的降解程度取决于细胞浓度和三氯乙烯浓度。大肠杆菌重组菌株对三氯乙烯的降解呈线性速率。相比之下，恶臭假单胞菌F1的突变株恶臭假单胞菌F39/D不含有顺式甲苯二氢二醇脱氢酶，其对三氯乙烯的初始降解速率要快得多，但会随时间下降。
相似文献
1
Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.含有克隆的恶臭假单胞菌F1甲苯双加氧酶基因的大肠杆菌对三氯乙烯的降解作用
Appl Environ Microbiol. 1989 Dec;55(12):3162-6. doi: 10.1128/aem.55.12.3162-3166.1989.
2
Stereospecific hydroxylation of indan by Escherichia coli containing the cloned toluene dioxygenase genes from Pseudomonas putida F1.含恶臭假单胞菌F1克隆甲苯双加氧酶基因的大肠杆菌对茚满的立体特异性羟基化作用。
Appl Environ Microbiol. 1992 Oct;58(10):3407-9. doi: 10.1128/aem.58.10.3407-3409.1992.
3
Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1.在恶臭假单胞菌F1的全细胞研究中，甲苯双加氧酶对三氯乙烯的降解作用。
Appl Environ Microbiol. 1988 Jul;54(7):1703-8. doi: 10.1128/aem.54.7.1703-1708.1988.
4
Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase.一种杂合芳香环双加氧酶对三氯乙烯的高效降解
J Bacteriol. 1994 Apr;176(7):2121-3. doi: 10.1128/jb.176.7.2121-2123.1994.
5
6
Oxidation of nitrotoluenes by toluene dioxygenase: evidence for a monooxygenase reaction.甲苯二加氧酶催化硝基甲苯的氧化：单加氧酶反应的证据。
Appl Environ Microbiol. 1992 Aug;58(8):2643-8. doi: 10.1128/aem.58.8.2643-2648.1992.
7
Toluene degradation by Pseudomonas putida F1: genetic organization of the tod operon.恶臭假单胞菌F1对甲苯的降解：tod操纵子的基因组织
Appl Environ Microbiol. 1988 Jun;54(6):1498-503. doi: 10.1128/aem.54.6.1498-1503.1988.
8
Trichloroethylene metabolism by microorganisms that degrade aromatic compounds.通过降解芳香化合物的微生物进行的三氯乙烯代谢
Appl Environ Microbiol. 1988 Feb;54(2):604-6. doi: 10.1128/aem.54.2.604-606.1988.
9
Cloning, sequencing, and expression of isopropylbenzene degradation genes from Pseudomonas sp. strain JR1: identification of isopropylbenzene dioxygenase that mediates trichloroethene oxidation.来自假单胞菌属菌株JR1的异丙苯降解基因的克隆、测序及表达：介导三氯乙烯氧化的异丙苯双加氧酶的鉴定
Appl Environ Microbiol. 1996 Nov;62(11):3967-77. doi: 10.1128/aem.62.11.3967-3977.1996.
10
Recruitment and expression of toluene/trichloroethylene biodegradation genes in bacteria native to deep-subsurface sediments.深层地下沉积物中天然细菌甲苯/三氯乙烯生物降解基因的招募与表达
Appl Environ Microbiol. 1996 Jul;62(7):2647-50. doi: 10.1128/aem.62.7.2647-2650.1996.
引用本文的文献
1
High-Affinity Chemotaxis to Histamine Mediated by the TlpQ Chemoreceptor of the Human Pathogen Pseudomonas aeruginosa.高亲和力组氨酸趋化作用由人类病原体铜绿假单胞菌的 TlpQ 化学感受器介导。
mBio. 2018 Nov 13;9(6):e01894-18. doi: 10.1128/mBio.01894-18.
2
Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System.对TodS/TodT信号转导系统中甲苯传感的分子见解。
J Biol Chem. 2016 Apr 15;291(16):8575-90. doi: 10.1074/jbc.M116.718841. Epub 2016 Feb 22.
3
Microbial Succession during a Field Evaluation of Phenol and Toluene as the Primary Substrates for Trichloroethene Cometabolism.在苯酚和甲苯作为三氯乙烯共代谢的主要基质的现场评估中微生物的演替。
Appl Environ Microbiol. 1997 Apr;63(4):1515-22. doi: 10.1128/aem.63.4.1515-1522.1997.
4
Horizontal transfer of phnAc dioxygenase genes within one of two phenotypically and genotypically distinctive naphthalene-degrading guilds from adjacent soil environments.在来自相邻土壤环境的两个表型和基因型不同的萘降解群落之一中，phnAc双加氧酶基因的水平转移
Appl Environ Microbiol. 2003 Apr;69(4):2172-81. doi: 10.1128/AEM.69.4.2172-2181.2003.
5
Characterization of the adaptive response to trichloroethylene-mediated stresses in Ralstonia pickettii PKO1.皮氏罗尔斯通氏菌PKO1中三氯乙烯介导的应激适应性反应的表征
Appl Environ Microbiol. 2002 Nov;68(11):5231-40. doi: 10.1128/AEM.68.11.5231-5240.2002.
6
Cytotoxicity associated with trichloroethylene oxidation in Burkholderia cepacia G4.洋葱伯克霍尔德菌G4中与三氯乙烯氧化相关的细胞毒性。
Appl Environ Microbiol. 2001 May;67(5):2107-15. doi: 10.1128/AEM.67.5.2107-2115.2001.
7
Succession of phenotypic, genotypic, and metabolic community characteristics during in vitro bioslurry treatment of polycyclic aromatic hydrocarbon-contaminated sediments.多环芳烃污染沉积物体外生物泥浆处理过程中表型、基因型和代谢群落特征的演替
Appl Environ Microbiol. 2001 Apr;67(4):1542-50. doi: 10.1128/AEM.67.4.1542-1550.2001.
8
Toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene, toluene, and trichloroethylene.降解甲苯的细菌对环境污染物苯、甲苯和三氯乙烯具有趋化性。
Appl Environ Microbiol. 2000 Sep;66(9):4098-104. doi: 10.1128/AEM.66.9.4098-4104.2000.
9
Induction of the tod operon by trichloroethylene in Pseudomonas putida TVA8.恶臭假单胞菌TVA8中三氯乙烯对tod操纵子的诱导作用。
Appl Environ Microbiol. 1998 Dec;64(12):5049-52. doi: 10.1128/AEM.64.12.5049-5052.1998.
10
Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria.甲苯氧化细菌对三氯乙烯降解的影响因素比较
Appl Environ Microbiol. 1996 Mar;62(3):825-33. doi: 10.1128/aem.62.3.825-833.1996.
本文引用的文献
1
Toxicity of Trichloroethylene to Pseudomonas putida F1 Is Mediated by Toluene Dioxygenase.三氯乙烯对恶臭假单胞菌 F1 的毒性是由甲苯双加氧酶介导的。
Appl Environ Microbiol. 1989 Oct;55(10):2723-5. doi: 10.1128/aem.55.10.2723-2725.1989.
2
Novel pathway of toluene catabolism in the trichloroethylene-degrading bacterium g4.三氯乙烯降解菌 g4 中甲苯代谢的新途径。
Appl Environ Microbiol. 1989 Jun;55(6):1624-9. doi: 10.1128/aem.55.6.1624-1629.1989.
3
Trichloroethylene biodegradation by a methane-oxidizing bacterium.甲烷氧化菌对三氯乙烯的生物降解。
Appl Environ Microbiol. 1988 Apr;54(4):951-6. doi: 10.1128/aem.54.4.951-956.1988.
4
Aerobic metabolism of trichloroethylene by a bacterial isolate.一株细菌分离株对三氯乙烯的需氧代谢。
Appl Environ Microbiol. 1986 Aug;52(2):383-4. doi: 10.1128/aem.52.2.383-384.1986.
5
THE DECOMPOSITION OF TOLUENE BY SOIL BACTERIA.土壤细菌对甲苯的分解作用
J Gen Microbiol. 1964 Jul;36:107-22. doi: 10.1099/00221287-36-1-107.
6
Purification and properties of NADH-ferredoxinTOL reductase. A component of toluene dioxygenase from Pseudomonas putida.NADH-铁氧还蛋白TOL还原酶的纯化及性质。恶臭假单胞菌甲苯双加氧酶的一个组分。
J Biol Chem. 1981 Mar 25;256(6):2723-30.
7
Oxidation of trichloroethylene by liver microsomal cytochrome P-450: evidence for chlorine migration in a transition state not involving trichloroethylene oxide.肝脏微粒体细胞色素P-450对三氯乙烯的氧化作用：氯在不涉及三氯乙烯环氧化物的过渡态中迁移的证据。
Biochemistry. 1982 Mar 2;21(5):1090-7. doi: 10.1021/bi00534a041.
8
Regulation of ribosomal RNA promoters with a synthetic lac operator.利用合成的乳糖操纵子调控核糖体RNA启动子
Proc Natl Acad Sci U S A. 1984 Nov;81(22):6929-33. doi: 10.1073/pnas.81.22.6929.
9
The aerobic pseudomonads: a taxonomic study.需氧假单胞菌属：一项分类学研究。
J Gen Microbiol. 1966 May;43(2):159-271. doi: 10.1099/00221287-43-2-159.
10
Formation of (+)-cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by Pseudomonas putida.恶臭假单胞菌由甲苯形成（+）-顺式-2,3-二羟基-1-甲基环己-4,6-二烯。
Biochemistry. 1970 Mar 31;9(7):1626-30. doi: 10.1021/bi00809a023.
Zotero 插件