Huang C J, Shaw H M
Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.
J Nutr. 1994 Apr;124(4):571-9. doi: 10.1093/jn/124.4.471.
In vitro erythrocyte hemolysis previously has been found to be significantly elevated in rats fed a low protein diet. To investigate the effects of dietary protein insufficiency on tissue vitamin E status, four groups of male weanling Long-Evans rats were given free access to either an 8% (wt/wt) lactalbumin diet containing 50 (L50) or 500 (L500) mg/kg diet of all-rac-alpha-tocopheryl acetate, or a 20% (wt/wt) lactalbumin diet containing 50 (N50) or 500 (N500) mg/kg of all-rac-alpha-tocopheryl acetate for 6 wk. A fifth group (P50) was pair-fed to the L50 group with the N50 diet. The L50 and L500 groups showed signs of protein malnutrition such as growth retardation as well as lower hematocrit and plasma protein and albumin concentrations. The alpha-tocopherol concentrations of plasma, heart, lung, kidney and muscle were significantly lower in the L50 group compared with the N50 group. The L500 group showed significantly less alpha-tocopherol in heart, kidney, muscle, brain and adipose tissue compared with the N500 group. The alpha-tocopherol concentrations in liver, testis, spleen and adrenal gland did not significantly differ either between the L50 and N50 groups or between the L500 and N500 groups. Supplementation with 500 mg/kg diet of all-rac-alpha-tocopheryl acetate resulted in significantly higher tissue alpha-tocopherol concentration in all tissues except muscle. The tissue alpha-tocopherol concentrations of the P50 group were not significantly different from those of the N50 group. The results indicated that protein insufficiency resulted in significantly lower alpha-tocopherol concentrations in most tissues in young growing rats.
先前已发现，喂食低蛋白饮食的大鼠体外红细胞溶血显著升高。为了研究膳食蛋白质不足对组织维生素E状态的影响，将四组雄性断奶Long-Evans大鼠自由喂食含50（L50）或500（L500）mg/kg饮食的全消旋α-生育酚醋酸酯的8%（重量/重量）乳白蛋白饮食，或含50（N50）或500（N500）mg/kg全消旋α-生育酚醋酸酯的20%（重量/重量）乳白蛋白饮食，持续6周。第五组（P50）与L50组配对喂食N50饮食。L50和L500组表现出蛋白质营养不良的迹象，如生长迟缓以及较低的血细胞比容、血浆蛋白和白蛋白浓度。与N50组相比，L50组血浆、心脏、肺、肾脏和肌肉中的α-生育酚浓度显著降低。与N500组相比，L500组心脏、肾脏、肌肉、大脑和脂肪组织中的α-生育酚显著减少。L50和N50组之间以及L500和N500组之间，肝脏、睾丸、脾脏和肾上腺中的α-生育酚浓度没有显著差异。补充500 mg/kg饮食的全消旋α-生育酚醋酸酯导致除肌肉外所有组织中的组织α-生育酚浓度显著升高。P50组的组织α-生育酚浓度与N50组没有显著差异。结果表明，蛋白质不足导致幼龄生长大鼠大多数组织中的α-生育酚浓度显著降低。
