Mikulskis A V, Cornelis G R
Microbial Pathogenesis Unit, Université Catholique de Louvain, UCL 54.90, Brussels, Belgium.
Mol Microbiol. 1994 Jan;11(1):77-86. doi: 10.1111/j.1365-2958.1994.tb00291.x.
YmoA and Hha are highly similar bacterial proteins downregulating gene expression in Yersinia enterocolitica and Escherichia coli, respectively. The phenotype of ymoA mutants evokes that of mutants affected in some histone-like proteins. This paper describes complementation of a ymoA mutation in Y. enterocolitica by the hha gene from E. coli. We show that YmoA and Hha are not only very similar proteins but that they are functionally interchangeable. Genetic experiments indicate that Hha can also stimulate transposition events in vivo. By Southern blot analysis we detected hha-homologous genes at least in Citrobacter diversus, Shigella flexneri, Shigella dysenteriae, Klebsiella pneumoniae and Salmonella typhimurium. We suggest that both YmoA and Hha belong to a new family of proteins downregulating gene expression in different enterobacteria.
YmoA和Hha是高度相似的细菌蛋白，分别在小肠结肠炎耶尔森菌和大肠杆菌中下调基因表达。ymoa突变体的表型与一些受组蛋白样蛋白影响的突变体相似。本文描述了大肠杆菌的hha基因对小肠结肠炎耶尔森菌ymoa突变的互补作用。我们发现YmoA和Hha不仅是非常相似的蛋白，而且在功能上可以互换。遗传学实验表明，Hha在体内也能刺激转座事件。通过Southern印迹分析，我们至少在奇异柠檬酸杆菌、福氏志贺菌、痢疾志贺菌、肺炎克雷伯菌和鼠伤寒沙门氏菌中检测到了hha同源基因。我们认为YmoA和Hha都属于一个在不同肠道细菌中下调基因表达的新蛋白家族。
