Metenier L, Grosclaude J, Meriaux J C
Dev Biol Stand. 1984;57:77-83.
The chief application of blood typing in domestic animals is in the verification of parentage. However, the acquisition of good standardized reagents in sufficient quantity remains an obstacle for the development of this work. The production of monoclonal antibodies directed against blood group determinants offers an attractive means of improving both the quality and quantity of serological reagents, and could facilitate the definition of new specificities. Fusions between a mouse myeloma line and splenocytes from mice immunized with horse red cells have resulted in four hybridomas producing antibodies against equine erythrocyte groups. Two are directed against the established groups Aa and Ca, while one reacts with a sub-group of De, and another, still under study, appears to be anti-Di. The anti-Aa and anti-Ca monoclonals have high affinity and fix complement, and are now in routine use as blood grouping reagents. This is remarkable since good antibodies to these specific sites are virtually impossible to obtain by allo-immunization. These results offer encouragement for the future production of monoclonal antibodies against red cell and lymphocyte antigens in the domestic species.
血型鉴定在家畜中的主要应用是用于亲子关系的验证。然而，获取足量优质的标准化试剂仍然是这项工作开展的一个障碍。针对血型决定簇生产单克隆抗体为提高血清学试剂的质量和数量提供了一种有吸引力的方法，并且有助于确定新的特异性。将小鼠骨髓瘤细胞系与用马红细胞免疫的小鼠脾细胞进行融合，已产生了4个针对马红细胞血型的杂交瘤。其中两个针对已确定的Aa和Ca血型，一个与De血型的一个亚群发生反应，另一个仍在研究中，似乎是抗Di血型的。抗Aa和抗Ca单克隆抗体具有高亲和力且能固定补体，目前已作为血型鉴定试剂常规使用。这很了不起，因为通过同种免疫几乎不可能获得针对这些特定位点的优质抗体。这些结果为未来在家畜物种中生产针对红细胞和淋巴细胞抗原的单克隆抗体提供了鼓励。
