Rudman D, Chawla R K
Am J Physiol. 1976 Jan;230(1):50-5. doi: 10.1152/ajplegacy.1976.230.1.50.
Lyophilized bovine, porcine, and human choroid plexuses contain .02-.09 U of antidiuretic activity per milligram. The antidiuretic factor in bovine choroid plexus was concentrated 100 times by extraction with acetic acid, fractional precipitation with acetone and ethyl ether, gel filtration, and paper chromatography. Resulting choroid plexus fraction IIgammaB2 was eluted from Sephadex G-25 in position corresponding to molecular weight between 750 and 3,500; its antidiuretic activity was destroyed by trypsin, performic acid, and thioglycollic acid, but was not affected by leucine aminopeptidase, carboxypeptidase A or B, or cyanogen bromide. HgammaB2 possesses antidiuretic, pressor, and oxytocic potencies (measured in anesthetized-hydrated rat, anesthetized rat, and isolated rat uterus, respectively) of 1.9, 0.5, and 0.1 U/mg, respectively.
冻干的牛、猪和人脉络丛每毫克含有0.02 - 0.9单位的抗利尿活性。牛脉络丛中的抗利尿因子通过用乙酸提取、用丙酮和乙醚分级沉淀、凝胶过滤和纸色谱法浓缩了100倍。得到的脉络丛组分IIγB2从Sephadex G - 25中洗脱出来，其位置对应于分子量在750至3500之间；其抗利尿活性被胰蛋白酶、过甲酸和巯基乙酸破坏，但不受亮氨酸氨肽酶、羧肽酶A或B或溴化氰的影响。HγB2分别在麻醉水合大鼠、麻醉大鼠和离体大鼠子宫中测得的抗利尿、升压和催产效力分别为1.9、0.5和0.1单位/毫克。
