Ueda M, Funasaka Y, Ichihashi M, Mishima Y
Department of Dermatology, Kobe University School of Medicine, Japan.
Br J Dermatol. 1994 Mar;130(3):320-4. doi: 10.1111/j.1365-2133.1994.tb02927.x.
It has been proposed that basic fibroblast growth factor (bFGF) is an autocrine growth factor of melanoma cells, in contrast with normal melanocytes where bFGF acts as a paracrine growth factor. As this notion is mostly based on the different requirements for bFGF in cultures of benign and malignant pigment cells in vitro, we performed an immunohistochemical study to examine bFGF expression in vivo, using paraffin sections from naevus cell naevi (NCN) and malignant melanoma (MM). All the NCN (n = 7) showed strong and homogeneous expression of bFGF protein, whereas the primary MMs (n = 5) showed heterogeneous expression, with a population of negative cells. Metastatic MMs (n = 5) also showed heterogeneous expression, and had a greater population of negative cells. These results suggest that bFGF has some, as yet unidentified, role in the growth of benign NCN, and that overexpression of bFGF is neither a prerequisite for melanoma genesis nor for progression to metastatic MM.
有人提出，碱性成纤维细胞生长因子（bFGF）是黑色素瘤细胞的自分泌生长因子，这与正常黑素细胞不同，在正常黑素细胞中bFGF作为旁分泌生长因子起作用。由于这一观点主要基于体外培养的良性和恶性色素细胞对bFGF的不同需求，我们进行了一项免疫组织化学研究，使用来自痣细胞痣（NCN）和恶性黑色素瘤（MM）的石蜡切片来检测体内bFGF的表达。所有的NCN（n = 7）均显示bFGF蛋白的强而均匀的表达，而原发性MM（n = 5）显示出异质性表达，存在一群阴性细胞。转移性MM（n = 5）也显示出异质性表达，且阴性细胞群体更多。这些结果表明，bFGF在良性NCN的生长中具有某种尚未明确的作用，并且bFGF的过表达既不是黑色素瘤发生的先决条件，也不是进展为转移性MM的先决条件。
