Mancianti M L, Györfi T, Shih I M, Valyi-Nagy I, Levengood G, Menssen H D, Halpern A C, Elder D E, Herlyn M
Wistar Institute of Anatomy and Biology, Hospital of the University of Pennsylvania, Philadelphia 19104.
J Invest Dermatol. 1993 Mar;100(3):281S-287S. doi: 10.1111/1523-1747.ep12470161.
Cells isolated from congenital melanocytic nevi and cultured in vitro have growth characteristics that resemble their premalignant stage in situ. A serum-free, chemically defined medium has been developed that allows continuous growth of established nevus cultures for up to several months. Like primary melanoma cells, nevus cells in high-calcium-containing W489 medium require insulin for growth. In contrast to melanoma cells, nevus cells in serum-free medium require the presence of alpha-melanocyte-stimulating hormone, which enhanced intracellular levels of cyclic adenosine monophosphate. In contrast to the requirements of normal human melanocytes from newborn foreskin, congenital nevus cells grow with less dependency on basic fibroblast growth factor (bFGF). Nevus cultures contain bFGF-like activity, and they express bFGF mRNA. Nevic cells of compound nevi also express bFGF mRNA in situ but only in the junctional areas. These results indicate that bFGF plays an important growth regulatory role for nevus cells in vitro and in vivo.
从先天性黑素细胞痣分离并在体外培养的细胞具有类似于其原位癌前阶段的生长特征。已开发出一种无血清、化学成分明确的培养基，可使已建立的痣细胞培养物持续生长长达数月。与原发性黑色素瘤细胞一样，在含高钙的W489培养基中的痣细胞生长需要胰岛素。与黑色素瘤细胞不同，无血清培养基中的痣细胞需要α-黑素细胞刺激素的存在，该激素可提高细胞内环磷酸腺苷的水平。与新生儿包皮正常人类黑素细胞的需求不同，先天性痣细胞生长对碱性成纤维细胞生长因子（bFGF）的依赖性较小。痣细胞培养物含有bFGF样活性，并且它们表达bFGF mRNA。复合痣的痣细胞在原位也表达bFGF mRNA，但仅在交界区域。这些结果表明，bFGF在体外和体内对痣细胞起着重要的生长调节作用。
