[一株产生CA125的人卵巢透明细胞癌细胞系的建立与鉴定]
[Establishment and characterization of a CA125-producing human ovarian clear cell carcinoma cell line].
作者信息
Sasa H, Ishii K, Hirata J, Kikuchi Y, Nagata I, Kawai T, Senoo A, Sugita M, Sugishita T, Tenjin Y
机构信息
Department of Obstetrics and Gynecology, National Defense Medical College, Tokorozawa, Japan.
出版信息
Hum Cell. 1993 Dec;6(4):279-86.
A human ovarian cancer cell line designated "KK" was established from ascites of a patient with ovarian clear cell carcinoma. This cell line was grown for more than 2 years and over 140 passages in medium RPMI1640 containing 10% FCS. Doubling time of this cell line at passage 70 was approximately 4 days and saturation density was 1.1 x 10(5)/cm2. Plating efficiency was approximately 23%. Chromosome analysis revealed aneuploidy with a model number of 67. PAS-positive substances were present in the cytoplasm. CA125 and SLX were detected in both the original tumor and the cultured cells. This cell line is less sensitive to cisplatin than KF cells and IC50 was 0.95 microM.
一种名为“KK”的人卵巢癌细胞系是从一名卵巢透明细胞癌患者的腹水中建立的。该细胞系在含有10%胎牛血清的RPMI1640培养基中培养了2年多，传代超过140次。该细胞系在第70代时的倍增时间约为4天，饱和密度为1.1×10(5)/cm2。接种效率约为23%。染色体分析显示为非整倍体，众数为67。细胞质中存在PAS阳性物质。在原发肿瘤和培养细胞中均检测到CA125和SLX。该细胞系对顺铂的敏感性低于KF细胞，IC50为0.95微摩尔。
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