Mitropoulos K A, Reeves B E, Miller G J
MRC Epidemiology and Medical Care Unit, Wolfson Institute of Preventive Medicine, Medical College of St Bartholomew's Hospital, London, UK.
Blood Coagul Fibrinolysis. 1993 Dec;4(6):943-51.
The prolonged incubation of dilute plasma on ice in the presence of added sulphatide vesicles or the long-chain saturated fatty acids (FA) stearic acid (C18:0) or behenic acid (C22:0) induced a concentration-dependent increase in factor VII coagulant activity (VIIc). The addition of FA at various ratios to human serum albumin showed the micellar non-bound pool to be responsible for this effect, FA bound to the high-affinity or low-affinity binding sites of albumin having no influence on VIIc. Plasma VIIc also increased following addition of behenate-enriched lipoprotein particles produced by incubation of the d < 1.006 g/ml lipoprotein fraction with this FA, or addition of lipoprotein remnants produced by pre-incubation of the d < 1.006 g/ml fraction with lipoprotein lipase. Long-chain saturated fatty acids in the interface of lipoprotein remnants, produced by the interaction of triglyceride-rich lipoprotein particles with lipoprotein lipase, appear to provide a surface that activates the contact system of coagulation and subsequently factor VII.
在添加硫化物囊泡或长链饱和脂肪酸（FA）硬脂酸（C18:0）或山嵛酸（C22:0）的情况下，将稀释血浆在冰上长时间孵育会导致凝血因子VII促凝活性（VIIc）呈浓度依赖性增加。以不同比例将FA添加到人血清白蛋白中表明，胶束未结合池是造成这种效应的原因，与白蛋白高亲和力或低亲和力结合位点结合的FA对VIIc没有影响。在将密度<1.006 g/ml的脂蛋白部分与该FA一起孵育产生的富含山嵛酸的脂蛋白颗粒添加后，或在将密度<1.006 g/ml部分与脂蛋白脂肪酶预孵育产生的脂蛋白残粒添加后，血浆VIIc也会增加。由富含甘油三酯的脂蛋白颗粒与脂蛋白脂肪酶相互作用产生的脂蛋白残粒界面中的长链饱和脂肪酸似乎提供了一个激活凝血接触系统并随后激活因子VII的表面。
