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通过逆转录酶和链终止抑制剂测定脑心肌炎病毒RNA的3'-末端核苷酸序列。

3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors.

作者信息

Zimmern D, Kaesberg P

出版信息

Proc Natl Acad Sci U S A. 1978 Sep;75(9):4257-61. doi: 10.1073/pnas.75.9.4257.

DOI:10.1073/pnas.75.9.4257
PMID:81487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336091/
Abstract

We have adapted the chain-termination method for determining the nucleotide sequence of DNA of Sanger, Nicklen, and Coulson [(1977) Proc. Natl. Acad. Sci. USA 74, 5463--5467] for use with reverse transcriptase (RNA-directed DNA nucleotidyltransferase) on RNA templates. With this method and using a primer (the octanucleotide pdT7rC) directed at the 3'-terminal poly(A), we have determined a sequence of 166 residues in the genomic RNA of the picornavirus encephalomyocarditis virus.

摘要

我们已采用桑格、尼克伦和库尔森用于测定DNA核苷酸序列的链终止法[（1977年）《美国国家科学院院刊》74卷，5463 - 5467页]，使其适用于以RNA为模板的逆转录酶（RNA指导的DNA核苷酸转移酶）。利用这种方法并使用针对3'端多聚（A）的引物（八聚体pdT7rC），我们测定了微小核糖核酸病毒脑心肌炎病毒基因组RNA中166个残基的序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/f422a87b2105/pnas00668-0197-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/04cf9b37ee84/pnas00668-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/a3ce94a6251a/pnas00668-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/ca438926b87c/pnas00668-0197-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/f422a87b2105/pnas00668-0197-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/04cf9b37ee84/pnas00668-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/a3ce94a6251a/pnas00668-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/ca438926b87c/pnas00668-0197-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e3b/336091/f422a87b2105/pnas00668-0197-c.jpg

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3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors.通过逆转录酶和链终止抑制剂测定脑心肌炎病毒RNA的3'-末端核苷酸序列。
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