Yamaguchi K, Nalesnik M A, Carr B I
Department of Transplant Surgery, University of Pittsburgh, School of Medicine, Pennsylvania 15241.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1993;64(6):361-5. doi: 10.1007/BF02915135.
In situ hybridization was performed to detect albumin mRNA in normal liver, liver cirrhosis, primary liver tumors and secondary liver neoplasms. In areas of normal liver, and liver cirrhosis, signals for albumin mRNA were present in hepatocytes, whereas no signals were seen in other cells such as endothelial and Küpffer cells, bile duct epithelium and smooth muscle cells. In 53 of 56 hepatocellular carcinomas signals were present in tumor cells but in eight cholangiocarcinomas and 14 metastatic adenocarcinomas from large bowel or pancreas, carcinoma cells were negative for albumin mRNA. In three metastatic tumors (from two neuroendocrine carcinomas and one gastric leiomyosarcoma), tumor cells contained no signals, while the surrounding hepatocytes showed diffuse grains. In 15 of the 84 specimens examined in situ hybridization was applied to routine formalin-fixed and paraffin-embedded blocks and strong signals were obtained for albumin mRNA. We conclude that in situ hybridization of human albumin is a valid tool in the differential diagnosis of hepatocellular carcinoma from cholangiocarcinomas and tumors metastatic to the liver.
采用原位杂交技术检测正常肝脏、肝硬化、原发性肝癌及继发性肝肿瘤中的白蛋白信使核糖核酸（mRNA）。在正常肝脏和肝硬化区域，白蛋白mRNA信号存在于肝细胞中，而在内皮细胞、库普弗细胞、胆管上皮细胞和平滑肌细胞等其他细胞中未见信号。在56例肝细胞癌中有53例肿瘤细胞存在信号，但在8例胆管癌以及14例来自大肠或胰腺的转移性腺癌中，癌细胞白蛋白mRNA呈阴性。在3例转移性肿瘤（2例神经内分泌癌和1例胃平滑肌肉瘤）中，肿瘤细胞无信号，而周围肝细胞呈现弥漫性颗粒。在所检测的84份标本中，有15份对常规福尔马林固定石蜡包埋组织块进行原位杂交，获得了白蛋白mRNA的强信号。我们得出结论，人白蛋白原位杂交是鉴别肝细胞癌与胆管癌及肝转移瘤的有效工具。
