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99mTc-NGA 肝脏显像即时冻干试剂盒的研制

[The preparation of 99mTc-NGA instant lyophilized kit for hepatic imaging].

作者信息

Guan C, Chen Q, Tan T

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1993 Dec;24(4):429-32.

PMID:8150449
Abstract

The preparation of one-step kit for the 99mTc labelled galactosyl-neo-glycoalbumin (NGA) using SnCl2 reduced method was reported in this paper. We developed a method of quality control and established the optimum scheme of technetium labelling on the basis of exploring the effects of varied labelled conditions on radiochemical purity. Our data indicates : (a) the kit is sterile, pyrogen-free and no toxic effects; (b) the operation is simple and fast; (c) its period of validity lasts no less than 3 months; (d) the radiochemical purity > 95%; (e) the stability of 99mTc-NGA in vitro > 4 hours; (f) the results of animal imaging and clinical probation are satisfactory.

摘要

本文报道了采用氯化亚锡还原法制备99mTc标记的半乳糖基新糖白蛋白(NGA)一步法试剂盒。我们开发了一种质量控制方法,并在探索不同标记条件对放射化学纯度影响的基础上,建立了锝标记的最佳方案。我们的数据表明:(a)该试剂盒无菌、无热原且无毒性作用;(b)操作简单快捷;(c)其有效期不少于3个月;(d)放射化学纯度>95%;(e)99mTc-NGA在体外的稳定性>4小时;(f)动物成像和临床试用结果令人满意。

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