采用柱切换高效液相色谱-柱后检测系统同时测定磷脂酰胆碱、胆固醇酯和三酰甘油的氢过氧化物。

Simultaneous determination of hydroperoxides of phosphatidylcholine, cholesterol esters and triacylglycerols by column-switching high-performance liquid chromatography with a post-column detection system.

作者信息

Akasaka K, Ohrui H, Meguro H

机构信息

Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.

出版信息

J Chromatogr. 1993 Dec 22;622(2):153-9. doi: 10.1016/0378-4347(93)80261-2.

DOI:10.1016/0378-4347(93)80261-2

PMID:8150864

Abstract

A method for the simultaneous determination of hydroperoxides of phosphatidylcholines (PC), triacylglycerols (TG) and cholesterol esters (CE) has been developed. A sample was separated into a combined TG and CE hydroperoxides fraction and a PC hydroperoxides fraction on a short silica column. The fractions were introduced into an ODS column and another silica column by a valve-switching device. The PC hydroperoxides were monitored by a post-column detection system with diphenyl-1-pyrenylphosphine, and the TG and CE hydroperoxides were monitored by another switching device. With this system, the hydroperoxides were determined at the picomole level within 32 min. Their detection limits were 2-4 pmol at a signal-to-noise ratio of 3, and the relative standard deviations of the peak areas were 1.6-3.1%. This method was successfully applied to determine lipid hydroperoxides in human plasma.

摘要

已开发出一种同时测定磷脂酰胆碱（PC）、三酰甘油（TG）和胆固醇酯（CE）氢过氧化物的方法。通过短硅胶柱将样品分离成TG和CE氢过氧化物的混合馏分以及PC氢过氧化物馏分。通过阀切换装置将这些馏分引入反相硅胶柱和另一根硅胶柱。用二苯基-1-芘基膦的柱后检测系统监测PC氢过氧化物，并用另一个切换装置监测TG和CE氢过氧化物。使用该系统，可在32分钟内测定皮摩尔水平的氢过氧化物。在信噪比为3时，其检测限为2-4皮摩尔，峰面积的相对标准偏差为1.6-3.1%。该方法成功应用于测定人血浆中的脂质氢过氧化物。