Veningerová M, Prachar V, Lukácsová M, Glasa J, Holomán J
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
J Chromatogr. 1993 Dec 22;622(2):274-7. doi: 10.1016/0378-4347(93)80277-b.
A method is presented for the isolation, separation and determination of sparteine and its metabolites in urine. The isolation is based on rapid extraction with dichloromethane and pentane in a glass separator. For the separation and determination, capillary gas chromatography with nitrogen-phosphorus detection was used. The recovery of the method ranged from 81.6% to 94.8%, and the limit of determination varied between 0.2 and 0.5 microgram ml-1. For quantification, 17-ethylsparteine was used as the internal standard.
本文介绍了一种分离、测定尿液中司巴丁及其代谢物的方法。分离基于在玻璃分液漏斗中用二氯甲烷和戊烷快速萃取。分离和测定采用带氮磷检测器的毛细管气相色谱法。该方法的回收率在81.6%至94.8%之间，测定限在0.2至0.5微克/毫升之间。定量时，使用17-乙基司巴丁作为内标。
