Daly J A, Korgenski E K, Munson A C, Llausas-Magana E
Primary Children's Medical Center, University of Utah School of Medicine, Salt Lake City 84113.
J Clin Microbiol. 1994 Feb;32(2):531-2. doi: 10.1128/jcm.32.2.531-532.1994.
The Strep A OIA (BioStar, Inc., Boulder, Colo.) rapid detection system is an intriguing technology that utilizes an immunoassay relying on changes in reflected light to directly detect group A streptococcal antigen from specimens. In this evaluation, 424 routine pediatric throat specimens and 20 simulated oropharyngeal specimens with added mucoid (M type 3, 18) strains were cultured and tested by the Strep A OIA. The respective sensitivities and specificities were as follows: Strep A OIA versus enhanced broth culturing, 84.2 and 95.7%; and streptococcus-SXT agar (BBL Microbiology Systems, Cockeysville, Md.) culturing versus enhanced broth culturing, 82.9 and 98.6%. The Strep A OIA is an 8-min, technologist-friendly, accurate technique with an 89.4% agreement with traditional culturing.
A群链球菌免疫层析分析(OIA,BioStar公司,科罗拉多州博尔德市)快速检测系统是一项引人关注的技术,它利用一种基于反射光变化的免疫测定法直接从标本中检测A群链球菌抗原。在本评估中,对424份常规儿科咽喉标本以及20份添加了黏液样(M3型、18型)菌株的模拟口咽标本进行培养,并采用A群链球菌OIA进行检测。各自的敏感性和特异性如下:A群链球菌OIA与强化肉汤培养法相比,分别为84.2%和95.7%;链球菌-SXT琼脂(BBL微生物系统公司,马里兰州科基斯维尔)培养法与强化肉汤培养法相比,分别为82.9%和98.6%。A群链球菌OIA是一项只需8分钟、对技术人员友好且准确的技术,与传统培养法的符合率为89.4%。