Cheema S S, Richards L, Murphy M, Bartlett P F
Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
J Neurosci Res. 1994 Feb 1;37(2):213-8. doi: 10.1002/jnr.490370207.
Leukemia inhibitory factor (LIF) has several characteristics of a neurotrophic factor for sensory neurons. Here we have investigated whether LIF also supports the survival of axotomised sensory neurons in vivo. Newborn rat pups received a unilateral sciatic nerve transection and the injury site was treated with gelfoam soaked in phosphate buffered saline (PBS), nerve growth factor (NGF), or LIF. Neuronal nucleoli in the L5 dorsal root ganglia were counted, appropriate corrections applied, and the resultant neuronal loss expressed as a percentage of the contralateral intact side. In animals where LIF was administered neuronal loss was significantly reduced: 2 days after LIF treatment neuronal loss was 19.5% compared to 43% in PBS-treated animals; 3 days after LIF treatment neuronal loss was 20.4% compared to 40.2% in PBS-treated animals; however, 7 days after LIF treatment there was no significant reduction in the number of neurons lost. The degree of rescue of sensory neurons in vivo by LIF was found to be similar to NGF, which was not surprising as both factors supported the survival of a similar population of sensory neurons in vitro. Rescue was not observed when LIF-containing gelfoam was placed away from the axotomised nerve, suggesting that LIF's action may be associated with its retrograde transport or direct signalling at the site of nerve injury.
白血病抑制因子（LIF）具有感觉神经元神经营养因子的若干特性。在此，我们研究了LIF在体内是否也能支持轴突切断的感觉神经元存活。新生大鼠幼崽接受单侧坐骨神经横断术，损伤部位用浸泡在磷酸盐缓冲盐水（PBS）、神经生长因子（NGF）或LIF中的明胶海绵处理。对L5背根神经节中的神经元核仁进行计数，进行适当校正，并将由此产生的神经元损失表示为对侧完整侧的百分比。在给予LIF的动物中，神经元损失显著减少：LIF治疗后2天，神经元损失为19.5%，而PBS处理的动物为43%；LIF治疗后3天，神经元损失为20.4%，而PBS处理的动物为40.2%；然而，LIF治疗后7天，损失的神经元数量没有显著减少。发现LIF在体内对感觉神经元的挽救程度与NGF相似，这并不奇怪，因为这两种因子在体外都支持相似群体的感觉神经元存活。当含LIF的明胶海绵放置在远离轴突切断神经的位置时，未观察到挽救作用，这表明LIF的作用可能与其逆行运输或在神经损伤部位的直接信号传导有关。
