Curtis R, Adryan K M, Zhu Y, Harkness P J, Lindsay R M, DiStefano P S
Regeneron Pharmaceuticals Inc., Tarrytown, New York 10591.
Nature. 1993 Sep 16;365(6443):253-5. doi: 10.1038/365253a0.
Ciliary neurotrophic factor (CNTF) promotes the survival of several populations of neurons, including sensory and motor neurons. Although CNTF is abundant in adult sciatic nerve, the mature protein lacks a signal sequence and is not secreted; therefore, it has been proposed to act as a lesion factor. The identification of a functional CNTF receptor revealed ligand-specific phosphorylation cascades and gene induction. However, it is not clear how these signal-transducing events are elicited in neuronal cell bodies that may be distant from the source of CNTF. We report here that CNTF can be retrogradely transported by adult sensory neurons. More importantly, sensory and motor neurons both show greatly increased transport of CNTF following peripheral nerve lesion. Axotomy-induced increases in retrograde transport of neurotrophic factors may be an important response of neuronal cell bodies during regeneration.
睫状神经营养因子（CNTF）可促进包括感觉神经元和运动神经元在内的多种神经元群体的存活。尽管CNTF在成年坐骨神经中含量丰富，但其成熟蛋白缺乏信号序列且不分泌；因此，有人提出它作为一种损伤因子发挥作用。功能性CNTF受体的鉴定揭示了配体特异性磷酸化级联反应和基因诱导。然而，尚不清楚这些信号转导事件是如何在可能远离CNTF来源的神经元细胞体中引发的。我们在此报告，成年感觉神经元可逆行运输CNTF。更重要的是，在周围神经损伤后，感觉神经元和运动神经元对CNTF的运输均显著增加。轴突切断诱导的神经营养因子逆行运输增加可能是神经元细胞体在再生过程中的一种重要反应。
