Solano-Flores L P, Rosas-Arellano M P, Ciriello J
Department of Physiology, Health Sciences Centre, University of Western Ontario, London, Canada.
Neurosci Lett. 1993 Dec 24;164(1-2):217-20. doi: 10.1016/0304-3940(93)90895-r.
Experiments were done in conscious rats to investigate the effect of i.c.v. infusions of hypertonic NaCl solutions on the induction of the protein Fos in the arcuate nucleus (Arc). Neurons containing Fos-like immunoreactivity were observed throughout the rostrocaudal extent of Arc after i.c.v. infusions of hypertonic saline solutions (337-744 mM). However, most of the labelled neurons were confined to the middle third of the nucleus, in the region of the dorsomedial and ventromedial subnuclei. Few, if any Fos-labelled neurons were observed in Arc of animals that received i.c.v. infusions of isotonic (142 mM) or mild hypertonic (173 mM) saline solutions or a hyperosmotic (660 mOsm/kg) saline solution of mannitol. No Fos-labelled neurons were found in the subfornical organ, although a few were observed scattered throughout the organum vasculosum laminae terminalis (OVLT) in all the animals studied. The density nor the distribution pattern of Fos-labelled neurons in OVLT was altered in animals receiving i.c.v. infusions of hypertonic saline or hyperosmotic solutions. These data demonstrate that Arc neurons are activated during a hypertonic saline challenge and suggest that Arc may function as a sodium-sensitive structure that is involved in body-fluid and circulatory homeostasis.
在清醒大鼠身上进行实验，以研究脑室内注入高渗氯化钠溶液对弓状核（Arc）中Fos蛋白诱导的影响。在脑室内注入高渗盐溶液（337 - 744 mM）后，在Arc的整个前后范围内均观察到含有Fos样免疫反应性的神经元。然而，大多数标记神经元局限于核的中间三分之一，即背内侧和腹内侧亚核区域。在接受脑室内注入等渗（142 mM）或轻度高渗（173 mM）盐溶液或甘露醇高渗（660 mOsm/kg）盐溶液的动物的Arc中，很少观察到Fos标记的神经元（如果有的话）。在穹窿下器中未发现Fos标记的神经元，尽管在所有研究的动物中，在终板血管器（OVLT）中观察到少数散在的Fos标记神经元。在接受脑室内注入高渗盐或高渗溶液的动物中，OVLT中Fos标记神经元的密度和分布模式均未改变。这些数据表明，在高渗盐刺激期间Arc神经元被激活，并提示Arc可能作为一个对钠敏感的结构，参与体液和循环稳态。
