Aoki T, Hämmerling U, De Harven E, Boyse E A, Old L J
J Exp Med. 1969 Nov 1;130(5):979-1001. doi: 10.1084/jem.130.5.979.
The representation of mouse alloantigens belonging to three systems, H-2, theta and TL, on the surface of cells from thymus, spleen, lymph nodes, and peritoneal cavity, was studied by electron microscopy with ferritin-labeled antibody. As expected from earlier serological data, TL was confined to thymocytes, theta was found on thymocytes and lymphocytes, and H-2 occurred to some extent on all cell types observed. On reticular cells, lymphocytes, plasma cells, and eosinophils, the majority of the cell surface was occupied by H-2; thymocytes had considerably less H-2, and erythrocytes and peritoneal macrophages least of all. In every instance the representation of antigen was discontinuous, the fraction of the cell surface covered being characteristic both of the antigen and of the type of cell. H-2 and theta provide a striking example of this; H-2 is present in far higher amounts on lymphocytes than on thymocytes, whereas the converse is true of theta. Within areas positive for H-2 or theta, protuberances of the surface membrane were often antigen-negative. A better definition of cell surface structure, gained from studies such as this, is necessary for further inquiry into how the cell surface is assembled, and into selective gene action in relation to cellular differentiation.
运用铁蛋白标记抗体的电子显微镜技术，研究了属于H-2、θ和TL这三个系统的小鼠同种异体抗原在胸腺、脾脏、淋巴结和腹腔细胞表面的表达情况。正如早期血清学数据所预期的那样，TL仅限于胸腺细胞，θ存在于胸腺细胞和淋巴细胞上，而H-2在观察到的所有细胞类型中都有一定程度的表达。在网状细胞、淋巴细胞、浆细胞和嗜酸性粒细胞上，大部分细胞表面被H-2占据；胸腺细胞的H-2含量少得多，红细胞和腹腔巨噬细胞的H-2含量最少。在每种情况下，抗原的表达都是不连续的，细胞表面被覆盖的部分对于抗原和细胞类型来说都是特征性的。H-2和θ就是一个显著的例子；淋巴细胞上的H-2含量远高于胸腺细胞，而θ的情况则相反。在H-2或θ呈阳性的区域内，表面膜的突起往往是抗原阴性的。通过这样的研究获得的对细胞表面结构的更清晰定义，对于进一步探究细胞表面是如何组装的以及与细胞分化相关的选择性基因作用是必要的。
